Abstract
It is well established that antibodies of the IgG isotype are important for resisting infections. The measurement of IgG levels in supernatants of pokeweed mitogen (PWM) stimulated peripheral blood lymphocytes (PBL) is widely used in clinical laboratories to assess B and T cell function, and similar cultures are routinely run in many laboratories to study the T cell subsets involved in the regulation of IgG synthesis by B cells. Although ELISA and RIA assays are widely used for the detection of IgG in culture supernatants, a sensitive, fast and automated assay would be advantageous.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Jolley, M. E. (1984). Principles of Particle Concentration Fluorescence Immunoassay. Pandex Laboratories.
Waldman, T. A., Duam, M., Broder, S., Blackman, M., Bleaseman, R. M. and Strober, W. (1974). Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinemia. Lancet, 2, 609.
Editor information
Editors and Affiliations
Copyright information
© 1988 S. B. Pal and the Contributors
About this chapter
Cite this chapter
Compas, M.B., Alevy, Y.G. (1988). Establishment of a Particle Concentration Fluorescence Immunoassay (PCFIA) for the Measurement of Human IgG in Culture Supernatants. In: Pal, S.B. (eds) Reviews on Immunoassay Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-10318-8_6
Download citation
DOI: https://doi.org/10.1007/978-1-349-10318-8_6
Publisher Name: Palgrave Macmillan, London
Print ISBN: 978-1-349-10320-1
Online ISBN: 978-1-349-10318-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)