D2 Dopamine Receptors: Isolation and Mechanism

  • Philip G. Strange
Part of the Satellite Symposia of the IUPHAR 10th International Congress of Pharmacology book series (SSNIC)


D2 dopamine receptors have been studied intensively over the past few years from a number of view points and much information has emerged about their structure and function. Many questions remain to be answered, however, and in the present chapter I shall discuss some of our recent studies aimed at understanding the following:
  1. (i)

    the constitution of the receptor

  2. (ii)

    the nature of the effectors influenced by the D2 dopamine receptor via G-proteins

  3. (iii)

    the possibility of D2 dopamine receptor heterogeneity.



Dopamine Receptor Thyrotrophin Release Hormone Inositol Phosphate Anterior Pituitary Cell Inositol Phospholipid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abbott, W.M. and Strange, P.G. (1985). Partial purification of dopamine D2 receptors using lectin affinity columns. Biosci. Rep., 5, 303–308.CrossRefPubMedGoogle Scholar
  2. Canonico, P.L., Valdenegro, C.A. and Macleod, R.M. (1983). The inhibition of phosphatidylinositol turnover: a possible post receptor mechanism for the prolactin secretion-inhibitory effect of dopamine. Endocrinology, 113, 7–14.CrossRefPubMedGoogle Scholar
  3. Canonico, P.L., Jarvis, W.D., Judd, A.M. and Macleod, R.M. (1986). Dopamine does not attenuate phosphoinositide hydrolysis in anterior pituitary cells. J. Endocrinol., 110, 389–393.CrossRefPubMedGoogle Scholar
  4. Cooper, D.M.F., Bier-Laning, C.M., Haiford, M.K., Ahlijanian, M.K. and Zahniser, N.R. (1986). Dopamine acting through D-2 receptors inhibits striatal adenylate cyclase by a GTP dependent process. Mol. Pharmacol., 29, 113–119.PubMedGoogle Scholar
  5. Enjalbert, A., Sladeczek, F., Guillon, G., Bertrand, P., Shu, C., Epelbaum, J., Garcia-Sainz, A., Jard, S., Lombard, C., Kordon, C. and Bockaert, J. (1986). Angiotensin II and dopamine modulate both cAMP and inositol phosphate production in anterior pituitary cells. J. Biol. Chem., 261, 4071–4075.PubMedGoogle Scholar
  6. Hall, J.M., Frankham, P.A. and Strange, P.G. (1983). Use of cholate/sodium chloride for solubilisation of brain D2 dopamine receptors. J. Neurochem., 41, 1526–1532.CrossRefPubMedGoogle Scholar
  7. Leonard, M.N., Macey, C. and Strange, P.G. (1987). Heterogeneity of D2 dopamine receptors in different brain regions. Biochem. J. in press.Google Scholar
  8. Niznik, H.B., Grigoriadis, D.E. and Seeman, P. (1986). Photoaffinity labelling of dopamine D2 receptors by [3H]azidomethyl spiperone. FEBS Letts., 209, 71–76.CrossRefGoogle Scholar
  9. Pizzi, M., D’Agostini, F., Da Prada, M., Spano, P.F. and Haefely, W.E. (1987). Dopamine D2 receptor stimulation decreases the inositol trisphosphate level in rat striatal slices. Eur. J. Pharmacol., 136, 263–264.CrossRefPubMedGoogle Scholar
  10. Schofield, J.G. (1983). Use of a trapped fluorescent indicator to demonstrate effects of thyioliberin and dopamine on cytoplasmic calcium concentrations in bovine anterior pituitary cells. FEBS. Letts., 159, 79–83.CrossRefGoogle Scholar
  11. Senogles, S.E., Amlaiky, N., Johnson, A.L. and Caron, M.G. (1986). Affinity chromatography of the anterior pituitary D2 dopamine receptor. Biochemistry, 25, 749–753.CrossRefPubMedGoogle Scholar
  12. Senogles, S.E., Benovic, J.L., Amlaiky, N., Unson, C., Milligan, G., Vinitsky, R., Spiegel, A.M. and Caron, M.G. (1987) The D2 dopamine receptor of anterior pituitary is functionally associated with a pertussis toxin sensitive guanine nucleotide binding protein. J. Biol. Chem., 262, 4860–4867.PubMedGoogle Scholar
  13. Simmonds, S.H. and Strange, P.G. (1985). Inhibition of inositol phospholipid breakdown by D2 dopamine receptors in dissociated bovine anterior pituitary cells. Neuroscience Letters, 60, 267–272.CrossRefPubMedGoogle Scholar
  14. Simmonds, S.H., Strange, P.G., Hall, A.W. and Taylor, R.J.K. (1986). Guanine nucleotide effects on agonist binding at D2 dopamine receptors in bovine anterior pituitary. Biochem. Pharmacol., 35, 731–735.CrossRefPubMedGoogle Scholar
  15. Sokoloff, P., Redouane, K., Brann, M., Martres, M.P. and Schwartz, J.C. (1985). [3H]D0710 discriminates guanine nucleotide sensitive and insensitive dopamine binding sites. Naunyn Schmiedeberg’s Archiv. Pharmacol., 329, 236–243.Google Scholar
  16. Stoof, J.C. and Verheijden, P.F.H.M. (1986). D-2 receptor stimulation inhibits cyclic AMP formation brought about by D-1 receptor stimulation in rat neostriatum but not nucleus accumbens. Eur. J. Pharmacol., 129, 205–206.CrossRefPubMedGoogle Scholar
  17. Wheatley, M., Hall, J.M., Frankham, P.A. and Strange, P.G. (1984). Improvement in conditions for solubilisation and characterisation of brain D2 dopamine receptors using various detergents. J. Neurochem., 43, 926–934.CrossRefPubMedGoogle Scholar
  18. Williamson, R.A., Worrall, S. and Strange, P.G. (1987). Partial purification of D2 dopamine receptors from bovine brain. Biochem. Soc. Trans. in press.Google Scholar
  19. Worrall, S., Williamson, R.A. and Strange, P.G. (1986). The purification of D2 dopamine receptors from bovine brain. Biochem. Soc. Trans., 14, 1138–1139.CrossRefGoogle Scholar

Copyright information

© Philip G. Strange 1988

Authors and Affiliations

  • Philip G. Strange
    • 1
  1. 1.The Biological LaboratoryThe UniversityCanterburyUK

Personalised recommendations