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Selection and Evaluation of Model Neurones for Investigation of Magnetic Field Effects

  • P. W. Schuetz
  • F. Tebling
Chapter
Part of the Keynes Seminars book series (KESE)

Abstract

In this chapter we report the isolation and preliminary evaluation of a novel neuroblastoma derived cell type of human origin. Fused with a suitable partner, the resulting hybridoma will be used for investigating possible effects of cyclical magnetic fields on the functional expression of neurones. For practical reasons we have at present restricted the number of parameters for evaluation to neuronal morphology, synapse formation, the presence of receptors to neurotransmitters, and to a limited extent the generation of action potentials in response to chemical and electrical stimulation.

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References

  1. Freshney, R. I. (1983). Culture of Animal Cells, Alan R. Liss, New York.Google Scholar
  2. Gillespie, J. S., Hamilton, D. N. and Hosie, R. J. (1970). The extraneuronal uptake and localisation of noradrenaline in the cat spleen and the effect on this of some drugs. J. Physiol., 206, 563–590.CrossRefGoogle Scholar
  3. Greene, L. A. (1977). Synthesis, storage and release of acetylcholine by a noradrenergic pheochromocytoma cell line. Nature, 268, 349–351.CrossRefGoogle Scholar
  4. Greene, L. A. and Tischler, A. S. (1976). Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. natn Acad. Sci., 73, 2424–2428.CrossRefGoogle Scholar
  5. Minna, J., Glazer, D. and Nirenberg, M. (1972). Genetic dissection of neural properties using somatic cell hybrids. Nature New Biology, 235, 225–231.CrossRefGoogle Scholar
  6. Nelson, P. G. and Lieberman, M. (1981). Excitable Cells in Tissue Culture, Plenum Press, New York and London.Google Scholar
  7. Pfundner, P., Schauenstein, K. and Wick, G. (1978). Argon Laser als Anregungsquelle fuer die Immunfluoreszenz, In Medizin Technik 78. Fachverlag Schiele and Schön GmBH., Berlin/BRD, Vol. 23, 99.Google Scholar
  8. Purves, D. and Lichtman, J. W. (1985). Principles of Neural Development, Sinauer Assoc, Sunderland, MA.Google Scholar
  9. Schuetz, P. W. (1986). Development of a system for investigating field effects on nerve cell growth. Ph.D. Thesis, University of Strathclyde, Glasgow.Google Scholar
  10. Schuetz, P. W., Barbenel, J. C., Paul, J. P., McCruden, A. B., Vasquez, R. and Tebling, F. (1985). Radio frequency stimulation of neuron type cells, in U. Carraro and C. Angelini (eds). Cell Biology and Clinical Management in Functional Electro-stimulation of Neurons and Muscles, Cleup Editore, Padova, pp. 33–36.Google Scholar
  11. Strecker, H., Hachmann, H. and Seidel, L. (1979). Extrem empfindliche quantitative Analysenmethoden. Chemikerzeitung, 103, No. 2, Verlag Chemie, Weinheim/BRD, 53–68.Google Scholar
  12. Urban, G. and Pirker, K. (1984). Duennschichtsonden fuer simultane Messung physiologischer Parameter am Menschen. Informationsversanstaltung. Proc. Conf. Medizin und Technik, Graz, Austria (published by Ministry of Science and Research (BMWF)), 123–125.Google Scholar

Copyright information

© Bioengineering Unit, University of Strathclyde 1988

Authors and Affiliations

  • P. W. Schuetz
  • F. Tebling

There are no affiliations available

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