Abstract
The goal of this current line of work was to develop quantitative autoradiographic methods to regionally quantify the binding properties (i.e. Kd and Bmax) of opiate ligands in morphine dependent and naive rat brain. Such regional studies were not possible utilizing binding techniques to brain membrane preparations because of the limited availability of tissue from dissection of small brain regions. Even with regional dissection techniques, the fine anatomical resolution available with autoradiography was sacrificed. With quantitative autoradiographic methods, one could ask whether the development of opiate tolerance/dependence occurred as a result of regulation of the affinity or concentration of opiate binding sites in any of one or several regions rich in opiate binding sites.
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References
Alexander, G.M., Schwartzman, R.J., Bell, R.D., Yu, J. and Renthal, A. (1981). Quantitative Measurement of Local Cerebral Metabolic Rate for Glucose Using Tritiated 2-Deoxyglucose. Brain Res., 223, 59–67.
Atweh, S.F. and Kuhar, M.J. (1977a). Autoradiographic Localization of Opiate Receptors in Rat Brain (II). Brainstem. Brain Res., 129, 1–12.
Atweh, S.F. and Kuhar, M.J. (1977b). Autoradiographic Localization of Opiate Receptors in Rat Brain (III). The Telencephalon Brain Res., 134, 393–405.
Geary, W.A. and Wooten, G.F. (1983). Quantitative Film Autoradiography of Opiate Agonist and Antagonist Binding in Rat Brain. J. Pharmacol. Exp. Ther., 225, 234–240.
Palacios, J.M., Niehoff, D.H., and Kuhar, M.J. (1981). Receptor Autoradiography with Tritium-Sensitive Film: Potential for Computerized Densitometry. Neurosci. Lett., 25, 101–105.
Penney, J.B., Pan, H.S., Young, A.B., Frey K.A., and Dauth, G.W. (1981). Quantitative Autoradiography of [3H] Muscimol Binding in Rat Brain. Science (Washington, D.C.), 214, 1036–1038.
Reivich, M., Jehle, J.W., Sokoloff, L. and Kety, S.S. (1969) Measurement of Regional Cerebral Blood Flow with Antipyrine- 14C in Awake Cats. J. Appl. Physiol., 27, 296–300.
Simon, E.J. and Hiller, J.M. (1978). The Opiate Receptors. Ann. Rev. Pharmacol. Toxicol., 18, 371–394.
Snyder, S.H. and Childers, S.R. (1979). Opiate Receptors and Opioid Peptides. Ann. Rev. Neurosci., 2, 35–64.
Unnerstall, J.R., Kuhar, M.J., Niehoff, D.L. and Palacios, J.M. (1981). Benzodiazepine Receptors are Coupled to a Subpopulation of Gamma-Aminobutyric Acid (GABA) Receptors: Evidence from a Quantitative Autoradiographic Study. J. Pharmacol. Exp. Ther., 218, 797–804.
Unnerstall, J.R., Niehoff, D.L., Kuhar, M.J. and Palacios, J.M. (1981). Quantitative Receptor Autoradiography using [3H] Ultrofilm: Applications to Multiple Benzodiazepine Receptors. J. Neurosci. Methods, 6, 59–73.
Young, W.S. and Kuhar, M.J. (1979). A New Method for Receptor Autoradiography: 3H Opioid Receptors in Rat Brain. Brain Res., 179, 155–170.
Zivin, J.A. and Waud, D.R. (1982). How to Analyze Binding, Enzyme, and Uptake Data: The Simplest Case, a Single Phase. Life Sci., 30, 1407–1422.
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© 1985 The Wenner-Gren Centre
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Wooten, G.F., Geary, W.A. (1985). Quantitative Autoradiography of Drug Binding Sites: Methodological Considerations. In: Agnati, L.F., Fuxe, K. (eds) Quantitative Neuroanatomy in Transmitter Research. Wenner-Gren Center International Symposium Series. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-08171-4_26
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DOI: https://doi.org/10.1007/978-1-349-08171-4_26
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