Abstract
In our laboratories we have in previous studies been able to develop methods for the determination of dopamine steady-ptate levels (nmol/g) and dopamine turnover rates (nmol/g x min-1 ) in discrete dopamine nerve terminal systems of the tuberculum olfactorium and the nucleus caudatus putamen (Agnati et al. 1979), using the tyrosine hydroxylase inhibition method for the studies on dopamine turnover rate. Microfluorimetrical quantitation of catecholamine fluorescence has also been made in the discrete catecholamine nerve terminal systems of the median eminence (Lofstrom et al. 1976a). Using the tyrosine hydroxylase inhibition method it was possible to determine rate constants and half-lives of CA in dopamine and noradrenaline nerve terminal systems of the median eminence (Lofstrom et al. 1976b). In all these studies an apparent monophasic decline of the catecholamine stores was obtained following injection of the tyrosine hydroxylase inhibitor α-methyl-dl-p-tyrosine methyl ester, suggesting the existence of mainly one pool of catecholamines.
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© 1985 The Wenner-Gren Centre
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Fuxe, K. et al. (1985). Quantitative Microfluorimetry and Semiquantitative Immunocytochemistry as Tools in the Analysis of Transmitter Identified Neurons. In: Agnati, L.F., Fuxe, K. (eds) Quantitative Neuroanatomy in Transmitter Research. Wenner-Gren Center International Symposium Series. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-08171-4_22
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DOI: https://doi.org/10.1007/978-1-349-08171-4_22
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