The Effects of BZD, DAT, DAB and CDA on Mitotic Chromosome Aneuploidy and the Nuclear Spindle

  • A. Lafi
  • E. M. Parry
  • N. Danford

Summary

Cultured Chinese hamster liver fibroblast cells were treated with BZD, DAT, DAB and CDA (Sample A), to detect the induction of mitotic spindle defects and aneuploidy. BZD, DAT and DAB induced spindle defects and/or aneuploidy.

Keywords

DMSO Fluorine Toxicology Diethylstilboestrol Colcemid 

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Notes

  1. Danford, N.D. (1984). Measurement of levels of aneuploidy in mammalian cells using a modified hypotonic treatment. Mutation Res. 139: 127–132PubMedGoogle Scholar
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  3. Parry, E.M., Danford, N. and Parry, J.M. (1982). Differential staining of chromosomes and spindle and its use as an assay for determining the effect of diethylstilboestrol on cultured mammalian cells. Mutation Res. 105: 243–252PubMedGoogle Scholar
  4. Parry, J.M., Danford, N. and Parry, E.M. (1984). In vitro techniques for the detection of chemicals capable of inducing mitotic chromosome aneuploidy. Alta, 11: 117–128Google Scholar
  5. Wissinger, W.L., Estervig, D.N. and Wang, R.J. (1981). A differential staining technique for simulataneous visualisation of mitotic spindle and chromosomes in mammalian cells. Stain Technology 56: 221–226PubMedGoogle Scholar

Copyright information

© United Kingdom Environmental Mutagen Society 1985

Authors and Affiliations

  • A. Lafi
    • 1
  • E. M. Parry
    • 1
  • N. Danford
    • 1
  1. 1.Genetic Toxicology Research Group, Department of GeneticsUniversity College of SwanseaSwanseaUK

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