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The Comparative Mutagenicity of 4-Dimethylaminoazobenzene and 4-Cyanodimethylaniline in Plate-incorporation Tests and Fluctuation Assays

  • D. Gatehouse
  • D. Wedd
  • K. Wharton

Abstract

This work was undertaken as part of the Second UKEMS Collaborative Mutagenicity Study. Two compounds were evaluated, using different in vitro microbial mutagenicity assays. 4-dimethylaminoazobenzene (DAB), a potent hepatocarcinogen, was included in the study to monitor variations in the source, preparation and quantity of S9 mix employed in short-term in vitro tests. 4-cyanodimethylaniline (CDA) was included as a structural analogue of DAB. This compound has been reported to be a bacterial mutagen in vitro (Ashby, et al., 1980). In this study, the standard Ames protocol (Ames et al., 1975) was compared with variations on the preincubation technique (Yahagi et al., 1975; Ashby et al., 1982) and the fluctuation assay (Gatehouse and Delow, 1979) for the detection of these two compounds.

Keywords

Bacterial Mutagen Comparative Mutagenicity Fluctuation Test Mutagenic Potency Plate Incorporation 
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Notes

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Copyright information

© United Kingdom Environmental Mutagen Society 1985

Authors and Affiliations

  • D. Gatehouse
    • 1
  • D. Wedd
    • 1
  • K. Wharton
    • 1
  1. 1.Glaxo Group Research LtdWareUK

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