The Testing of four UKEMS Trial Compounds for Bacterial Mutation in the Salmonella/Microsome Assay Using (1) Aroclor-induced Rat Liver, (2) Chicken Liver S9 and (3) The York Modification of the Standard Ames Protocol
The testing of four UKEMS trial compounds in the presence of Aroclor 1254-induced rat liver S9 using the standard Ames protocol showed that BZD and DAT induced mutation in S. typhimurium TA1538. A modification of the standard testing procedure known as the ‘York method’ demonstrated enhanced mutagenicity with BZD but not with DAT; Sample B of CDA was rendered a mutagen using this procedure. Optimum mutagenicity was seen with a final S9 concentration of 10%. Chicken liver S9 was less effective than rat in activating the two positive mutagens.
KeywordsChicken Liver Positive Mutagen Standard Testing Procedure Microsomal Activation Cancer Research Campaign
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