Variation of the T-system in different types of intrafusal muscle fibre of cat spindles
A modified staining technique by Forbes & Sperelakis (1980) for demonstrating membrane systems of lizard skeletal muscles has been applied to investigate the occurrence of these systems in cat intrafusal muscle fibres. The technique requires a fixative consisting of glutaraldehyde, dextrose and dextran (40,000–90,000MW), all at 3% in an aqueous solution, with 50mM calcium chloride added, pH 7.4. Post-fixation is completed in a solution of 2% osmium tetroxide and 0.8% potassium ferrocyanide in 0.1M sodium cacodylate, then followed by block-staining in a saturated uranyl acetate aqueous solution.
KeywordsFibril Dextran Triad Odium Dehyde
Unable to display preview. Download preview PDF.
- Banks, R.W. (1981). A histological study of the motor innervation of the cat’s muscle spindle. J. Anat., 133, 571–591.Google Scholar
- Banks, R.W., Barker, D., Harker, D.W. and Stacey, M.J. (1975). Correlation between ultrastructure and histochemistry of mammalian intrafusal muscle fibres. J. Physiol. (Lond.), 252, 16–17P.Google Scholar
- Banks, R.W., Harker, D.W. and Stacey, M.J. (1977). A study of mammalian intrafusal muscle fibres using a combined histochemical and ultrastructural technique. J. Anat., 123. 783–796.Google Scholar
- Barker, D. (1974). The morphology of muscle receptors. In Handbook of Sensory Physiology 3 (ed. C.C. Hunt), 1–190. Springer-Verlag, Berlin.Google Scholar
- Boyd, I.A. (1962). The structure and innervation of the nuclear bag muscle fibre system and the nuclear chain muscle fibre system in mammalian muscle spindles. Phil. Trans. Roy. Soc. (Lond.) B, 245, 81–136.Google Scholar
- Forbes, M.S. and Sperelakis, N. (1980). Membrane systems in skeletal muscle of the lizard Anolis carolinensis. J. Ultrastruct. Res., 73, 245–261.Google Scholar
- Gladden, M.H. (1976). Structural features relative to the function of intrafusal muscle fibres in the cat. Prog. Brain Res., 44, 51–59.Google Scholar