The detection of drugs by histochemical procedures

  • M. Morley


Many methods of analysis are available to the toxicologist for determining the type and amount of drug within human tissues, but few have the potential of histochemistry for enabling the precise site of action of a drug to be defined. Histochemistry is essentially the examination of the chemical as opposed to the morphological structure of tissue preparations. Nondiffusible coloured or fluorescent reaction products which can be visualised under the microscope indicate the cellular localisation of the endogenous or exogenous substances under investigation.


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  1. Allen, J. (1963). Immunofluorescence applied to protein solutions. J. Lab. Clin. Med. 62, 517–24.PubMedGoogle Scholar
  2. Balkon, J., Bidanset, J. H., and Lynch, V. D. (1980). Immunofluorescence detection of drugs in postmortem assessment of drug influence in cause of death. J. Forens. Sci. 25, 88–94.CrossRefGoogle Scholar
  3. Böttiger, L. E. (1955a). On the distribution of tetracycline in the body. Antibiotics Chemother. 5, 332–9.Google Scholar
  4. Böttiger, L. E. (1955b). On the distribution of chlortetracycline in the body. Acta. Med. Scand. 151, 343–8CrossRefPubMedGoogle Scholar
  5. Chang, J. P., and Hori, S. H. (1961). The section freeze-substitution technique. 1: Method. J. Histochem. Cytochem. 9, 292–300.CrossRefGoogle Scholar
  6. Coons, A. H., and Kaplan, M. H. (1950). Localisation of antigen in tissue cells. 2: Improvements in a method for the detection of antigen by means of fluorescent antibody. J. Exper. Med. 91, 1–13.CrossRefGoogle Scholar
  7. Egorin, M. J., Hildebrand, R. C., Cimino, E. F., and Bachur, N. R. (1974). Cytofluorescence localization of adriamycin and daunorubicin. Cancer Res. 34, 2243–5.PubMedGoogle Scholar
  8. Feteanu, A. (1978a). Special technology and applications. In Labelled Antibodies in Biology and Medicine, 2nd edn. Abacus, Tunbridge Wells, p. 94.Google Scholar
  9. Feteanu, A. (1978b), Ibid., pp. 95–114.Google Scholar
  10. Gee, D. J., Gelder, S. R., Morley, M., and Sivaloganathan, S. (1981). Applications of microspectrofluorimetry to forensic medicine. UV Spectrometry Group Bulletin 9, 53–63.Google Scholar
  11. Gelder, S. R. (1978). A cold stage for observing an autoluminescent drug in tissue sections at 77 K using a DIALUX microscope. Leitz. M. H. Wiss v Techn. 8, No. 7.Google Scholar
  12. Gelder, S. R., and Gee, D. J. (1979). The visualisation of two autofluorescent drugs in tissue sections by fluorescent microscopy. J. Forens. Sci. Soc. 19, 107–116.CrossRefGoogle Scholar
  13. Helander, S. (1949). On the distribution of some salicylic acid derivatives in the tissues. Acta. Pharmacol. 6, 97–106.CrossRefGoogle Scholar
  14. Helander, S., and Böttiger, L. E. (1953). On the distribution of terramycin in different tissues. Acta. Med. Scand. 47, 71–5.Google Scholar
  15. Kaplan, M. H. (1958). Immunologic studies of heart tissue. 1: production in rabbits of antibodies reactive with an autologous myocardial antigen following immunisation with heterologous heart tissue. J. Immun. 80, 254–67.PubMedGoogle Scholar
  16. Kohn, K. W. (1961). Mediation of divalent metal ions in the binding of tetracycline to macromolecules. Nature, Lond. 191, 1156–8.CrossRefPubMedGoogle Scholar
  17. Liss, R. H., and Norman, J. C. (1975). Visualisation of doxycycline in lung tissue and sinus secretions by fluorescent techniques. Chemotherapy 21 (suppl. 1), 27–35.CrossRefPubMedGoogle Scholar
  18. Maestrone, G. (1963). Demonstration of leptospiral and viral antigens in formalin-fixed tissues. Nature. Lond. 197, 409–10.CrossRefGoogle Scholar
  19. Morley, M., and Gee, D. J. (1982). An assessment of the immunofluorescence technique as a method of demonstrating the histological localisation of tetrahydrocannabinol in post-mortem tissues. J. Forens. Sci. 27, 837–43.CrossRefGoogle Scholar
  20. Morley, M., Gee, D. J., Moffat, A., and Gaunt, P. J. (1982). The effect of proteins on drug autofluorescence: forensic applications. In preparation.Google Scholar
  21. Nairn, R. C. (1976a). Immunological tracing: general considerations. In Fluorescent Protein Tracing, 4th edn. Churchill Livingstone, Edinburgh and New York, pp. 132–4; 142–5.Google Scholar
  22. Nairn, R. C. (1976b). Ibid., p. 158.Google Scholar
  23. Pearse, A. G. E. (1968a). Fluorescent methods (immunofluorescence). In Histochemistry: Theoretical and Applied. 3rd edn. Williams and Wilkins. Baltimore. vol. 1. p. 203.Google Scholar
  24. Pearse, A. G. E. (1968b). The chemistry of fixation. Ibid., vol. 1, pp. 86–8.Google Scholar
  25. Pearse, A. G. E. (1968c). Cold knife and microtome methods. Ibid., vol. 1. p. 25.Google Scholar
  26. Pearse, A. G. E. (1968d). Fluorescent antibody methods (immunofluorescence). Ibid., vol. 1. pp. 188–95.Google Scholar
  27. Pearse, A. G. E. (1972). Substrate film techniques. Ibid., vol. 2, pp. 1009–15.Google Scholar
  28. Pertschuk, L. P., and Sher, J. H. (1975). Demonstration of methadone in the human brain by immunofluorescence. Res. Commun. Chem. Pathol. Pharmacol. 11, 319–22.PubMedGoogle Scholar
  29. Pertschuk, L. P., Ford, D. H., Rainfold, E. A., and Brigati, D. J. (1976). Localization of phenobarbital in mouse central nervous system by immunofluorescence. Acta Neurol. Scand. 53, 325–34.CrossRefPubMedGoogle Scholar
  30. Pertschuk, L. P., Ford, D. H., and Rainford, E. A. (1977). Localization of methadone in fetal rat eye by the immunofluorescence technic. Expl. Eye Res. 24, 547–52.CrossRefGoogle Scholar
  31. Stora, C. (1980a). Histofluorescence technique for the intracellular localization of chemical carcinogens. Histochem. J. 12, 631–40.CrossRefPubMedGoogle Scholar
  32. Stora, C. (1980b). Cellular localization of chemical carcinogens studied by fluorescence microscopy. Oncology 37, 20–2.CrossRefPubMedGoogle Scholar
  33. Stora, C. (1980c). Comparative study of the cellular localization of three polycylic hydrocarbons differing in their carcinogenicity. Ibid. 37, 23–6.CrossRefPubMedGoogle Scholar
  34. Udenfriend, S. (1962). Fluorescence Assay in Biology and Medicine. Academic, New York, vol. 1.Google Scholar

Further Reading Affinity Chromatography

  1. Affinity Chromatography, Principles and Methods(undated). Pharmacia Fine Chemicals, Uppsala.Google Scholar


  1. (a) General principles Udenfriend, S. (1962). Fluorescence Assay in Biology and Medicine, Academic,New York, San Francisco, London, vol 1.Google Scholar
  2. (b) Microscopy Nairn, R. C. (1976). Fluorescence microscopy and photomicography. In Fluorescent Protein Tracing, 4th edn. Churchill Livingstone, Edinburgh, pp. 68–108.Google Scholar
  3. Pearse, A. G. E. (1972). Fluorescence microscopy. In Histochemistry, Theoretical and Applied, 3rd edn. Churchill Livingstone, Edinburgh, vol. 2, pp. 1171–206.Google Scholar
  4. Ploem, J. S. (1977). Quantitative fluorescence microscopy. In Meek, G. A., and Elder, H. Y. (eds), Analytical and Quantitative Methods in Microscopy, 1st edn. Cambridge University Press, Cambridge, pp. 55–90.Google Scholar


  1. Pearse, A. G. E. (1968). Histochemistry, Theoretical and Applied, 3rd edn. Williams and Wilkins, Baltimore, vol. 1.Google Scholar
  2. Pearse, A. G. E. (1972). Ibid., vol. 2.Google Scholar

Immunoenzyme techniques

  1. Feteanu, A. (1978). Labelled Antibodies in Biology and Medicine. Abacus, Tunbridge Wells.Google Scholar
  2. Heyderman, E. (1979). Immunoperoxidase technique in histopathology: applications, methods and controls. J. Clin. Pathol. 32, 971–8.CrossRefPubMedPubMedCentralGoogle Scholar

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  • M. Morley

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