Effects of cycloleucine on mitochondrial RNA

  • Donald T Dubin
  • Christine M Green
  • Daniel L Prince


Mitochondria contain their own genetic system, which specifies not only the mRNA’s for a particular set of proteins but also the ribosomal and transfer RNA’s required for translating these mRNA’s. In mammalian mitochondria the ribosomal and most transfer RNA’s are methylated, as would be expected for rRNA and tRNA, but in distinctive fashion. The rRNA’s have very few methylated residues, 5 per molecule for the small subunit (“13S”) RNA and 3 per molecule for the large subunit (“17S”) RNA, or about 20 to 50% as many as the homologous E. coli prototypes (Dubin & Taylor, 1978; Dubin et al, 1978). The tRNA contains about 2 methylated residues on the average per molecule, a figure which resembles that for E. coli and is 1/3 that for cytoplasmic tRNA; its methylated nucleoside composition constitutes a subset of that of cytoplasmic (cyt) tRNA, only m3C, m5C, m1A, m1G, m2G, and m22G being present in significant amounts (Dubin & Friend, 1974; Davenport et al, 1976).


Methionine Metabolism Mammalian Mitochondrion Standard Salt Apparent Degree Density Gradient Sedimentation 
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  1. Amalric, F., Bachellerie, J-P., and Caboche, M. (1977). RNA Methylation and Control of Eukaryotic RNA Biosynthesis: Processing and Utilization of Undermethylated tRNA’s in CHO Cells. Nucl. Acids Res., 4, 4357–4370.PubMedPubMedCentralCrossRefGoogle Scholar
  2. Attardi, G. (1981). Organization and Expression of the Mitochondrial Genome: a Lesson in Economy. TIBS, 6, 100–103.Google Scholar
  3. Caboche, M. (1977). Methionine Metabolism in BHK Cells: The Regulation of Methionine Adenosyltransferase. J. Cell Physiol., 92, 407–424.PubMedCrossRefGoogle Scholar
  4. Caboche, M., and Bachellerie, J-P. (1977). RNA Methylation and Control of Eukaryotic RNA biosynthesis. Effects of Cycloleucine, a Specific Inhibitor of Methylation, on Ribosomal RNA Maturation. Eur. J. Biochem., 74, 19–29.PubMedCrossRefGoogle Scholar
  5. Caboche, M. and Hatzfeld, J. (1978). Methionine Metabolism in BHK Cells: Preliminary Characterization of the Physiological Effects of Cycloleucine, an Inhibitor of S-adenosylmethionine Biosynthesis. J. Cell Physiol., 97, 361–370.PubMedCrossRefGoogle Scholar
  6. Davenport, L.W., Taylor, R.H. nad Dubin, D.T. (1976). Comparison of Human and Hamster Mitochondrial Transfer RNA: Physical Properties and Methylation Status. Biochim. Biophys. Acta, 447, 285–293.Google Scholar
  7. Dubin, D.T. (1974). Methylated Nucleotide Content of Mitochondrial Ribosomal RNA from Hamster Cells. J. Mol. Biol., 84, 257–273.PubMedCrossRefGoogle Scholar
  8. Dubin, D.T. and Friend, D.A. (1972). Comparison of Cytoplasmic and Mitochondrial 4S RNA from Cultured Hamster Cells: Physical and Metabolic Properties. J. Mol. Biol., 71, 163–175.PubMedCrossRefGoogle Scholar
  9. Dubin, D.T. and Friend, D.A. (1974). Methylation Properties of Mitochondrion-Specific Transfer RNA from Cultured Hamster Cells. Biochim. Biophys. Acta, 340, 269–277.PubMedCrossRefGoogle Scholar
  10. Dubin, D.T., Taylor, R.H. and Davenport, L.W. (1978). Methylation Status of 13S Ribosomal RNA from Hamster Mitochondria: The Presence of a Novel Riboside, N4-methylcytidine. Nucl. Acids Res., 4, 4385–97.Google Scholar
  11. Dubin, D.T. and Taylor, R.H. (1978). Modification of Mitochondrial Ribosomal RNA from Hamster Cells: The Presence of GmG and Latemethylated UmGmU in the Large Subunit (17S) RNA. J. Mol. Biol. 121, 523–540.PubMedCrossRefGoogle Scholar
  12. Eperon, I.C., Anderson, S. and Nierlich, D.P. (1980). Distinctive Sequence of Human Mitochondrial Ribosomal RNA Genes. Nature, 286, 460–467.PubMedCrossRefGoogle Scholar
  13. Green, C.M. and Dubin, D.T. (1980). The Effect of Cycloleucine on Methylation of Mitochondrial RNA in Hamster Cells. Fed. Proc., 38, 2201.Google Scholar
  14. HsuChen, C-C. and Dubin, D.T. (1980). Methylation Patterns of Mycoplasma Transfer and Ribosomal RNA. J. Bacteriol., 144, 991–998.PubMedPubMedCentralGoogle Scholar
  15. Randerath, D., Agrawal, H.P. and Randerath, E. (1981). Tumor Mitochondrial Transfer RNAs: The Nucleotide Sequence of Mitochondrial Morris Hepatoma 5123D. Biochem. Biophys. Res. Comm., 100, 723–737.Google Scholar
  16. Sodd, M.A. (1976). Analysis of the Primary and Secondary Structure of tRNA. In Handbook of Biochemistry and Molecular Biology 3rd Ed., Nucleic Acids Vol. II (ed. G.D. Fasman), CRC Press, Cleveland, pp. 432–456.Google Scholar

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© The contributors 1982

Authors and Affiliations

  • Donald T Dubin
  • Christine M Green
  • Daniel L Prince

There are no affiliations available

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