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In vitro Screening Tests for Anthelmintics

  • D. C. Jenkins
Chapter

Abstract

Few rational parameters exist for the selection of new compounds for evaluation as potential anthelmintics. Therefore, dependence is often placed on an empirical approach where it is essential that the investigator screen as wide a variety of chemical types as possible. According to Neal (1980), to stand a good statistical chance of success, about 50 new compounds need to be evaluated each week. Clearly, to achieve this goal a simple, rapid but relevant first-stage in vitro screen consuming no more than l-5mg of compound would be far more practicable than any animal model. Such in vitro tests are used frequently and very effectively in the search for new antiprotozoal drugs (Neal, 1980), but the majority of anthelmintic screens still rely on the use of long-established animal models. In fact, the use of in vitro screens for anthelmintics has been criticised, often quite irrationally, in the past and there seems to have been some reluctance to expend resources on their study and improvement.

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References

  1. Berntzen, A. K. (1965). Comparative growth and development of Trichinella spiralis in vitro and in vivo, with a redescription of the life cycle. Expl Parasit., 16, 74–106CrossRefGoogle Scholar
  2. Campbell, W. C. and Blair, L. S. (1974). Chemotherapy of Trichinella spiralis infections (a review). Expl Parasit., 35, 304–34CrossRefGoogle Scholar
  3. Chen, S. N. and Howells, R. E. (1979). The in vitro cultivation of the infective larvae and the early mammalian stages of the filarial worm, Brugia pahangi. Ann. trop. Med. Parasit., 73, 473–86Google Scholar
  4. Coles, G. C. and McNeillie, R. M. (1977). The response of nematodes in vivo and in vitro to some anthelmintics. J. Helminth., 51, 323–6CrossRefGoogle Scholar
  5. Denham, D. A., Suswillo, R. R., Rogers, R. and Nelson, G. S. (1978). Studies with Brugia pahangi. 20. An investigation of 23 anthelmintics using different screening techniques. Trans. R. Soc. trop. Med. Hyg., 72, 615–8CrossRefGoogle Scholar
  6. Diamond, L. S. and Douvres, F. W. (1962). Bacteria-free cultivation of some parasitic stages of the swine nematodes Hyostrongylus rubidus and Oesophagostomum quadrispinulatum (O. longicaudum). J. Parasit., 48, 39–42CrossRefGoogle Scholar
  7. Henson, P. M., Mackenzie, C. D. and Spector, W. G. (1979). Inflammatory reactions in onchocerciasis: a report on current knowledge and recommendations for further study. Bull. Wld Hlth Org., 57, 667–82Google Scholar
  8. Jenkins, D. C., Armitage, R. and Carrington, T. S. (1980). A new primary screening test for anthelmintics utilizing the parasitic stages of Nippostrongylus brasiliensis, in vitro. Z. ParasitKde, 63, 261–9CrossRefGoogle Scholar
  9. Jenkins, D. C. and Carrington, T. S. (1981). An in vitro screening test for compounds active against the parenteral stages of Trichinella spiralis. Tropenmed. Parasit., 32 (in press)Google Scholar
  10. Keeling, J. E. D., Lewis, G. and Jenkins, D. C. (1980). A modified medium for the in vitro cultivation of trichostrongylid nematodes and its use for anthelmintic screening. Trans. R. Soc. trop. Med. Hyg., 74, 679 [Abstract]Google Scholar
  11. Leland, S. E., Ridley, R. K., Slonka, G. F. and Zimmerman, G. L. (1975). Detection of activity for various anthelmintics against in vitro-produced Cooperia punctata. Am. J. Res. vet. Sci., 36, 449–56Google Scholar
  12. Meerovitch, E. (1965). Studies on the in vitro axenic development of Trichinella spiralis. 1. Basic culture techniques, pattern of development, and the effects of the gaseous phase. Can. J. Zool., 43, 69–79CrossRefGoogle Scholar
  13. Neal, R. A. (1980). In vitro screening systems and antiprotozoal agents. In The Use of Alternatives in Drug Research (A. N. Rowan and C. J. Stratman, eds), Macmillan Press, LondonGoogle Scholar
  14. Pudney, M. and Varma, M. G. R. (1980). Present state of knowledge of in vitro cultivation of filariae. In The in vitro Cultivation of the Pathogens of Tropical Diseases, WHO Tropical Diseases Research Series No. 3, Schwabe, BasleGoogle Scholar
  15. Simpkin, K. G. and Coles, G. C. (1981). The use of Caenorhabditis elegans for anthelmintic screening. J. chem. Tech. Biotech., 31, 66–9CrossRefGoogle Scholar
  16. Tiner, J. D. (1958). A preliminary in vitro test for anthelmintic activity. Expl Parasit., 7, 292–305CrossRefGoogle Scholar

Copyright information

© The contributors 1982

Authors and Affiliations

  • D. C. Jenkins
    • 1
  1. 1.Department of ParasitologyWellcome Research LaboratoriesBeckenham, KentUK

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