Abstract
Time-resolved electrophysiological studies of end-plate responses to acetylcholine and cholinergic agonists define the time range for molecular studies in which the events at the neuromuscular junction may be simulated in vitro. Since this time range is of the order of milliseconds, rapid kinetic techniques have to be employed. Suitable ligands for such studies require the following combination of properties: (1) they must have suitable spectroscopic properties, such as fluorescence with high quantum yield, and excitation and emission remote from the ultraviolet-absorption range of proteins; (2) their pharmacological properties must closely resemble those of the natural transmitter and, in particular, must be uncomplicated by side effects which may obscure interpretation of kinetic data.
This is a preview of subscription content, log in via an institution to check access.
Preview
Unable to display preview. Download preview PDF.
References
Fertuck, H. C. and Salpeter, M. M. (1976). J. Cell Biol., 69, 144–58
Heidmann, T. and Changeux, J.-P. (1978). A. Rev. Biochem., 47, 317–57
Jürss, R. and Maelicke, A. (1980). J. biol. Chem., in press
Jürss, R., Prinz, H. and Maelicke, A. (1979a). Proc. natn. Acad. Sci. U.S.A., 76, 1064–8
Jürss, R., Prinz, H. and Maelicke, A. (1979b). In Recent Advances in Receptor Chemistry (ed. F. Gualtieri, M. Gianella and C. Melchiorre), Elsevier/North Holland Biomedical Press, Amsterdam, pp. 59–70
Klett, R.P., Fulpius, B.W., Cooper, D., Smith, M., Reich, E. and Possani, L. D. (1973). J. biol. Chem., 248, 6841–53
Lentz, T. L., Mazurkiewicz, J. and Rosenthal, J. (1977). Brain Res., 132, 423–42
Maelicke, A., Fulpius, B. W., Klett, R. P. and Reich, E. (1977). J. biol. Chem., 252, 4811–30
Mathews-Bellinger, J. and Salpeter, M. M. (1978). J. Physiol., Lond., 279, 197–213
Nachmansohn, D. (1975). Chemical and Molecular Basis of Nerve Activity, Academic Press, New York
Neumann, E., Rosenberry, T. L. and Chang, H. W. (1978). In Neuronal Information Transfer (ed. A. Karlin et al.). Academic Press, New York, pp. 183–210
Prinz, H. and Maelicke, A. (1980). To be published
Rogers, A. W., Darzynkiewicz, Z., Salpeter, M. M., Ostrowski, K. and Barnard, E. A. (1969). J. Cell Biol., 41, 665–85
Rüchel, R., Watters, D. and Maelicke, A. (1980). To be published.
Salpeter, M. M., Plattner, H. and Rogers, A. W. (1972). Histochem. Cytochem., 20, 1059–68
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Copyright information
© 1981 Institute of Biology Endowment Trust Fund
About this chapter
Cite this chapter
Prinz, H., Jürss, R., Maelicke, A. (1981). Interaction of fluorescent analogues of acetylcholine with nicotinic acetylcholine receptors and acetylcholine esterase. In: Birdsall, N.J.M. (eds) Drug Receptors and Their Effectors. Biological Council. Palgrave, London. https://doi.org/10.1007/978-1-349-05555-5_9
Download citation
DOI: https://doi.org/10.1007/978-1-349-05555-5_9
Publisher Name: Palgrave, London
Print ISBN: 978-1-349-05557-9
Online ISBN: 978-1-349-05555-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)