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Stable Isotopes pp 15–26Cite as

Palgrave Macmillan

Mass spectrometric methods of isotope determination

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Abstract

Although there are several analytical techniques for determining the isotopic composition of a sample, combined gas chromatography—mass spectrometry (GC—MS) is often the most suitable for analysis of samples of biological origin (Watson, 1976). For example, biological samples are rarely pure (even after extensive sample processing) and the biologically active component may represent only a trace constituent of the total sample. The combination of a separation technique with an identification technique in GC—MS permits a complex mixture to be separated in the time domain so that characteristic mass spectra can be obtained from picomole to nanomole quantities of sample depending on the mode of instrument operation.

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References

  • Änggård, E. and Sedvall, G. (1969). Anal. Chem., 41, 1250

    Article  Google Scholar 

  • Axen, U., Gréen, K., Horlin, D. and Samuelsson, B. (1971). Biochem. Biophys. Res. Commun., 45, 519

    Article  Google Scholar 

  • Biller, J. W. and Biemann, K. (1974). Anal. Lett., 7, 515

    Article  Google Scholar 

  • Braselton, W. E., Orr, J. C. and Engel, L. L. (1973). Anal. Biochem., 53, 64

    Article  Google Scholar 

  • Bush, M. T., Sekerke, H. J. Jr., Vore, M., Sweetman, B. J. and Watson, J. T. (1972). Proceedings of Seminar on the Use of Stable Isotopes in Clinical Pharmacology (ed. P. D. Klein and L. J. Roth), Technical Information Center, CONF-711115, Springfield, Va., USA, p. 233

    Google Scholar 

  • Falkner, F. C., Sweetman, B. J. and Watson, J. T. (1975). Appl. Spectrosc. Rev., 10, 51

    Article  Google Scholar 

  • Farmer, J. B. (1963). Mass Spectrometry (ed. C. A. McDowell), McGraw-Hill, New York, Chapter 2, p. 7

    Google Scholar 

  • Frölich, J. C., Wilson, T. W., Sweetman, B. J., Nies, A. S., Carr, K., Watson, J. T. and Oates, J. A. (1975). J. Clin. Invest., 55, 763

    Article  Google Scholar 

  • Gréen, K. (1969). Chem. Phys. Lipids, 3, 254

    Article  Google Scholar 

  • Hammar, C.-G., Alexanderson, B., Holmstedt, B. and Sjöqvist, F. (1971). Clin. Pharmacol. Therap., 12, 496

    Google Scholar 

  • Hammar, C.-G., Holmstedt, B. and Ryhage, R. (1968). Anal. Biochem., 25, 532

    Article  Google Scholar 

  • Heath, E. C., Falkner, F. C., Hill, R. E. and Watson, J. T. (1974). Proceedings of the 22nd Annual Conference on Mass Spectrometry and Allied Topics, Philadelphia, American Society for Mass Spectrometry, p. 286

    Google Scholar 

  • Hites, R. A. and Biemann, K. (1967). Anal. Chem., 39, 965

    Article  Google Scholar 

  • Hites, R. A. and Biemann, K. (1970). Anal. Chem., 42, 855

    Article  Google Scholar 

  • Hoffenberg, R., Lawson, A. M., Ramsden, D. R. and Raw, P. J. (1973). Mass Spectrometry in Biochemistry and Medicine (ed. A. Frigerio and N. Castagnoli), Raven Press, New York, p. 303

    Google Scholar 

  • Holmes, W. F., Holland, W. H. and Parker, J. A. (1971). Anal. Chem., 43, 1806

    Article  Google Scholar 

  • Holmstedt, B. and Lindgren, J.-E. (1974). The Poisoned Patient: The Role of the Laboratory, Ciba Foundation Symposium 26, ASP (Elsevier—Exerpta Medica—North-Holland), Amsterdam, p. 106

    Google Scholar 

  • Holmstedt, B. and Palmér, L. (1973). Advan. Biochem. Psychopharmacol., 7, 1

    Google Scholar 

  • Knapp, D. R., Gaffney, T. W. and McMahon, R. E. (1972). Biochem. Pharmacol., 21, 425

    Article  Google Scholar 

  • Pohl, L. R., Nelson, S. D., Garland, W. A. and Trager, W. F. (1975). Biomed. Mass Spectrom., 2, 23

    Article  Google Scholar 

  • Samuelsson, B., Hamberg, M. and Sweeley, C. C. (1970). Anal. Biochem., 33, 301

    Article  Google Scholar 

  • Sweeley, C. C., Elliott, W. H., Fries, I. and Ryhage, R. (1966). Anal. Chem., 38, 1549

    Article  Google Scholar 

  • Sweeley, C. C., Ray, B. D., Wood, W. K., Holland, J. F. and Krichevsky, M. I. (1970). Anal. Chem., 42, 1505

    Article  Google Scholar 

  • Vore, M., Sweetman, B. J. and Bush, M. T. (1974). J. Pharmacol. Exp. Ther., 190, 384

    Google Scholar 

  • Waller, G. R. (1972). Biochemical Applications of Mass Spectrometry, Wiley-Interscience, New York

    Google Scholar 

  • Watson, J. T. (1976). Introduction to Mass Spectrometry for Biomedical, Environmental and Forensic Application, Raven Press, New York

    Google Scholar 

  • Watson, J. T., Falkner, F. C. and Sweetman, B. J. (1974). Biomed. Mass Spectrom., 1, 156

    Article  Google Scholar 

  • Watson, J. T., Pelster, D. R., Sweetman, B. J., Frölich, J. C. and Oates, J. A. (1973). Anal. Chem., 45, 2071

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Vanderbilt University, Nashville, Tenn. 37232, USA

    J. Throck Watson

Authors
  1. J. Throck Watson
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Editor information

Editors and Affiliations

  1. Department of Clinical Pharmacology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK

    T. A. Baillie

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© 1978 The Contributors

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Watson, J.T. (1978). Mass spectrometric methods of isotope determination. In: Baillie, T.A. (eds) Stable Isotopes. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-03328-7_2

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