Adenylate cyclase: actions and interactions of regulatory ligands

  • C. Londos
  • M. Rodbell
Part of the Biological Council book series (BCSDA)

Abstract

The discovery of adenylate cyclase systems has provided a means for study of hormone action in vitro under controlled conditions. By 1960 it had been established by Sutherland and his associates (Sutherland and Rall, 1960) that the only ingredients necessary to elicit a hormonal effect on the enzyme were magnesium ion, ATP, hormone and membranes bearing the enzyme. This seemingly simple system, in which hormone was shown to increase the Vmax of adenylate cyclase, soon became considerably more complex when it was shown that several of the basic assay components exerted multiple effects on the enzyme. While such effects have contributed to a confusing body of literature on adenylate cyclase, they have led also to the elucidation of certain regulatory features of the enzyme system. In this report we shall present a brief review of the current ideas and controversies regarding the interaction of several regulatory ligands with adenylate cyclase, followed by some more recent data which may help to resolve at least one of these controversies.

Keywords

Fluoride Adenine Pyrimidine Neuroblastoma Purine 

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Copyright information

© Institute of Biology Endowment Trust Fund 1977

Authors and Affiliations

  • C. Londos
    • 1
  • M. Rodbell
    • 1
  1. 1.Membrane Regulation Section, Laboratory of Nutrition and Endocrinology, National Institute of Arthritis, Metabolism and Digestive DiseasesNational Institutes of HealthBethesdaUSA

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