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In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in Drosophila melanogaster

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Circular RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2765))

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Abstract

Studying circular RNAs’ function in vivo has been challenging due to the lack of generic tools to manipulate their levels without affecting their linear counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdown with tissue-specific resolution in flies.

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Acknowledgments

Research reported in this publication was supported by NIH grants R01GM122406 and AG057700 to SK. We thank Dr. Ane Martin Anduaga for comments and discussions.

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Author notes

  1. Ines Lucia Patop and Michael Canori contributed equally.

Authors and Affiliations

  1. Brandeis University, Waltham, MA, USA

    Ines Lucia Patop, Michael Canori & Sebastian Kadener

Authors
  1. Ines Lucia Patop
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  2. Michael Canori
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  3. Sebastian Kadener
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Corresponding author

Correspondence to Sebastian Kadener .

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Editors and Affiliations

  1. Klaus Tschira Institute for Integrative Computational Cardiology, University Heidelberg, Heidelberg, Germany

    Christoph Dieterich

  2. Department of Cellular, Computational and Integrative Biology – CIBIO, University of Trento, Trento, Italy

    Marie-Laure Baudet

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Patop, I.L., Canori, M., Kadener, S. (2024). In Vivo Tissue-Specific Knockdown of circRNAs Using shRNAs in Drosophila melanogaster. In: Dieterich, C., Baudet, ML. (eds) Circular RNAs. Methods in Molecular Biology, vol 2765. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3678-7_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3678-7_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3677-0

  • Online ISBN: 978-1-0716-3678-7

  • eBook Packages: Springer Protocols

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