Abstract
Organoid technology, as a three-dimensional (3D) culture method, provides a feasible tool to self-organize multiple types of organ-specific cells, construct inherent anatomic structures, and display functional biological activities. For the purpose of renal regeneration, renal organoids are considered as predictive options to form functional kidney substitutions in vitro. Here, we describe an accessible and convenient way to generate renal organoids without differentiation procedures using whole kidney cells.
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Acknowledgments
This work was performed at Wake Forest Institute for Regenerative Medicine (Wake Forest University, Winston-Salem, North Carolina, USA) and supported by funds from the chief of institute, Anthony Atala.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Chen, L. (2024). Renal Organoids from Whole Kidney Cells. In: Sumbalova Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 2764. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3674-9_11
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DOI: https://doi.org/10.1007/978-1-0716-3674-9_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3673-2
Online ISBN: 978-1-0716-3674-9
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