Abstract
Distinct bands of mucins cannot be banded using a gel electrophoresis based on a molecular sieving effect due to their very large molecular weight and remarkable diversity in glycosylation. In contrast, membrane electrophoresis can separate mucins as round bands. Here, we present an analysis of mucin separation via membrane electrophoresis using a porous polyvinylidene difluoride membrane, which is highly stable against chemical modifications and various organic solvents. The separated mucins can not only be stained with dyes but also with antibodies and lectins, and glycans can be released from the excised bands and analyzed.
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Acknowledgments
This work was supported by a Grant-in-Aid for Scientific Research (KAKENHI 23310154, 17H03808, 17K19627, and 21H02123) from the Japan Society for the Promotion of Science (JSPS).
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Kameyama, A. (2024). Supported Molecular Matrix Electrophoresis. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_7
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DOI: https://doi.org/10.1007/978-1-0716-3670-1_7
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3670-1
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