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Fabrication and Characterization of Mucin Nanoparticles for Drug Delivery Applications

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Mucins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2763))

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Abstract

Mucin glycoproteins are ideal biomacromolecules for drug delivery applications since they naturally offer a plethora of different functional groups that can engage in specific and unspecific binding interactions with cargo molecules. However, to fabricate drug carrier objects from mucins, suitable stabilization mechanisms have to be implemented into the nanoparticle preparation procedure that allow for drug release profiles that match the requirements of the selected cargo molecule and its particular mode of action. Here, we describe two different methods to prepare crosslinked mucin nanoparticles that can release their cargo either on-demand or in a sustained manner. This method chapter includes a description of the preparation and characterization of mucin nanoparticles (stabilized either with synthetic DNA strands or with covalent crosslinks generated by free radical polymerization), as well as protocols to quantify the release of a model drug from those nanoparticles.

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Acknowledgements

This project was funded by the Federal Ministry of Education and Research (BMBF) and the Free State of Bavaria under the Excellence Strategy of the Federal Government and the Länder through the ONE MUNICH Project Munich Multiscale Biofabrication.

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Authors and Affiliations

  1. School of Engineering and Design, Department of Materials Engineering, Technical University of Munich, Garching, Germany

    Ceren Kimna, Theresa M. Lutz & Oliver Lieleg

  2. Center for Protein Assemblies (CPA) and Munich Institute of Biomedical Engineering, Technical University of Munich, Garching, Germany

    Ceren Kimna, Theresa M. Lutz & Oliver Lieleg

Authors
  1. Ceren Kimna
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  2. Theresa M. Lutz
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  3. Oliver Lieleg
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Corresponding author

Correspondence to Oliver Lieleg .

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Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Akihiko Kameyama

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kimna, C., Lutz, T.M., Lieleg, O. (2024). Fabrication and Characterization of Mucin Nanoparticles for Drug Delivery Applications. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_33

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  • DOI: https://doi.org/10.1007/978-1-0716-3670-1_33

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3669-5

  • Online ISBN: 978-1-0716-3670-1

  • eBook Packages: Springer Protocols

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