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Preparation of Soluble Mucin Solutions from the Salivary Glands

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Mucins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2763))

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Abstract

Studying salivary gland mucins is important for elucidating the pathogenesis of salivary gland diseases, including tumors and xerostomia, and developing diagnostic methods for them. Classic methods for isolating mucins from salivary glands require sacrificing several animals to obtain sufficient quantities of mucin and are time-consuming. Supported molecular matrix electrophoresis (SMME) was used to characterize mucins and their glycans. With this method, mucins can be analyzed within 2 days using less than 100 mg of tissue and without using expensive equipment, such as an ultracentrifuge. This chapter describes a method for preparing mucin solutions for SMME analysis of salivary gland mucins.

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Author information

Authors and Affiliations

  1. Division of Oral and Maxillofacial Surgery, Ushiku Aiwa General Hospital, Ushiku, Japan

    Takanori Sugiura

  2. Department of Oral Oncology, Oral and Maxillofacial Surgery, Ichikawa General Hospital, Tokyo Dental College, Ichikawa, Japan

    Takanori Sugiura

  3. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

    Akihiko Kameyama

Authors
  1. Takanori Sugiura
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  2. Akihiko Kameyama
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Corresponding author

Correspondence to Akihiko Kameyama .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Akihiko Kameyama

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sugiura, T., Kameyama, A. (2024). Preparation of Soluble Mucin Solutions from the Salivary Glands. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3670-1_3

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3669-5

  • Online ISBN: 978-1-0716-3670-1

  • eBook Packages: Springer Protocols

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