Abstract
It is a challenging task to quantify mucin using conventional protein quantification methods due to the large number of glycans attached to the peptide, which make up approximately 50–90% of its molecular weight. To address this issue, we propose a simple quantification method that involves spotting mucins onto a membrane and staining them with Alcian blue.
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References
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Acknowledgments
The authors thank Mr. Shohei Kamei and Ms. Moeko Nakajima for their technical assistance. This work was supported by research grants from the Teikyo University and Josai University.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Tamura, M., Arata, Y. (2024). Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane. In: Kameyama, A. (eds) Mucins. Methods in Molecular Biology, vol 2763. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3670-1_10
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DOI: https://doi.org/10.1007/978-1-0716-3670-1_10
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3669-5
Online ISBN: 978-1-0716-3670-1
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