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Transfection of Heat-Shock Protein 70 Enhances Myocardial Tolerance to Ischemia: Evidence for a Role of HSP70 in Ischemia-Reperfusion Injury

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The Ischemic Heart

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 1))

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Abstract

The enhancement of myocardial tolerance mediated by heat-shock protein 70 (HSP70) can be utilized for further advancement in myocardial protection in clinical settings. Recently, we have developed a novel in vivo gene transfection method for the entire heart. We investigated the possibility of enhancing myocardial tolerance to ischemia-reperfusion injury by introducing the HSP70 gene into the whole heart by means of in vivo gene transfection with HVJ (Hemagglutinating virus of Japan)-liposome procedure. HVJ-liposome, either with the human HSP70 gene (H group; n = 5) or without the gene (C group; n = 5), was infused into rat hearts via the coronary arteries. The hearts obtained from nontreated rats (N group; n = 5) were also examined. Western blotting analysis clearly showed overexpression of HSP70 in the H group. Recovery rate of left ventricular developed pressure, rate-pressure product, and coronary flow after ischemia-reperfusion injury (37°C, 30 minutes) were significantly higher for the H group than for either the C or N group (p < 0.05). CK leakage for the first five minutes of reperfusion was lower in the H group than in the C and N groups (p < 0.05). HSP70 was overexpressed in rat hearts as a result of in vivo gene transfection with HVJ-liposome. Higher myocardial tolerance to ischemia-reperfusion injury was observed in the HSP70-overexpressing heart as compared to the control and even the nontreated hearts. Our results demonstrate the protective effect of gene-transfection-induced HSP70 against ischemia-reperfusion injury in the myocardium, suggesting the possibility of clinical application of gene therapy with HSP70 to ischemia-reperfusion injury.

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Authors and Affiliations

  1. Osaka University, Japan

    Yoshiki Sawa, Ken Suzuki, Yasufumi Kaneda, Keishi Kadoba, Hong-Zhi Bai, Koji Kagisaki, Ryuichi Morishita & Hikaru Matsuda

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  1. Yoshiki Sawa
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  2. Ken Suzuki
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  3. Yasufumi Kaneda
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  4. Keishi Kadoba
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  5. Hong-Zhi Bai
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  6. Koji Kagisaki
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  7. Ryuichi Morishita
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  8. Hikaru Matsuda
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Editor information

Editors and Affiliations

  1. Department of Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Seibu Mochizuki M.D., Ph.D. (Professor and Chairman) (Professor and Chairman)

  2. Department of Internal Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Nobuakira Takeda M.D., Ph.D. (Associate Professor) (Associate Professor)

  3. Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano M.D., Ph.D. (Professor-Emeritus) (Professor-Emeritus)

  4. MRC Group in Experimental Cardiology Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.), D.Sc. (Hon.) (Distinguished Professor and Director) (Distinguished Professor and Director)

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© 1998 Kluwer Academic Publishers

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Sawa, Y. et al. (1998). Transfection of Heat-Shock Protein 70 Enhances Myocardial Tolerance to Ischemia: Evidence for a Role of HSP70 in Ischemia-Reperfusion Injury. In: Mochizuki, S., Takeda, N., Nagano, M., Dhalla, N.S. (eds) The Ischemic Heart. Progress in Experimental Cardiology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-39844-0_31

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  • DOI: https://doi.org/10.1007/978-0-585-39844-0_31

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7923-8105-1

  • Online ISBN: 978-0-585-39844-0

  • eBook Packages: Springer Book Archive

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