Abstract
Anabaena PCC 7120 heterocysts undergo at least three programmed DNA rear-rangements that affect the nitrogen-fixation genes nifD and fdxN, and the uptake hydrogenase gene hupL RT-PCR showed that hupL is expressed only after heterocysts have formed. An open reading frame that encodes a product similar to hupS or hupU is upstream of hupL. A 10.5-kb DNA element interrupts the hupL open reading frame and is excised during heterocyst differentiation. Correct expression of the presumed hupSL operon requires both transcriptional regulation and excision of the element. The hupL element encodes its own site-specific recombinase, XisC, which is 46% identical to the predicted XisA recombinase present on the PCC 7120 nifD element. The XisA and XisC proteins show sequence similarity to the catalytic domains of the integrase family of site-specific recombinases and contain the absolutely conserved catalytic Tyr residue. However, the Anabaena recombinases represent a distinct subset of the integrase family.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Argos, P., Landy, A., Abremski, K., Egan, J.B., Haggard-Ljungquist, E., Hoess, R.H., Kahn, M.L., Kalionis, B., Narayana, S.V.L., Pierson III, L.S., Sternberg, N., and Leong, J.M., 1986, The integrase family of site-specific recombinases: regional similarities and global diversity, EMBO J., 5:433–440.
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J. A., and Struhl, K., 1994, Current protocols in molecular biology, Greene Publishing Associates and Wiley-Interscience, New York.
Black, L.K., Fu, C., and Maier, R.J., 1994, Sequences and characterization of hupU and hupV genes of Bradyrhizobium japonicum encoding a possible nickel-sensing complex involved in hydrogenase expression, J. Bacteriol., 176:7102–7106.
Carrasco, C.D., Buettner, J.A., and Golden, J.W., 1995, Programmed DNA rearrangement of a cyanobacterial hupL gene in heterocysts, in Proceedings of the National Academy of Sciences (USA), 92:791–795.
Carrasco, C.D., Ramaswamy, K.S., Ramasubramanian, T.S., and Golden, J.W., 1994, Anabaena xisF gene encodes a developmentally regulated site-specific recombinase, Genes Dev., 8:74–83.
Chen, J.W., Lee, J., and Jayaram, M., 1992, DNA cleavage in trans by the active site tyrosine during Flp recombination: switching protein partners before exchanging strands, Cell, 69:647–658.
Elhai, J., and Wolk, C.P., 1990, Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena, EMBO J., 9:3379–3388.
Elsen, S., Colbeau, A., Chabert, J., and Vignais, P.M., 1996, The hupTUV operon is involved in negative control of hydrogenase synthesis in Rhodobacter capsulatus, J. Bacteriol., 178:5174–5181.
Golden, J.W., Mulligan, M.E., and Haselkorn, R., 1987, Different recombination site specificity of two developmentally regulated genome rearrangements, Nature (London), 327:526–529.
Golden, J.W., Robinson, S.J., and Haselkorn, R., 1985, Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena, Nature (London), 314:419–423.
Golden, J.W., Whorff, L.L., and Wiest, D.R., 1991, Independent regulation of nifHDK operon transcription and DNA rearrangement during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120, J. Bacteriol., 173:7098–7105.
Han, Y.W., Gumport, R.I., and Gardner, J.F., 1994, Mapping the functional domains of bacteriophage lambda integrase protein, J. Mol. Biol., 235:908–25.
Kwon, H.J., Tirumalai, R., Landy, A., and Ellenberger, T., 1997, Flexibility in DNA recombination—structure of the lambda integrase catalytic core, Science, 276:126–131.
Leclerc, M., Colbeau, A., Cauvin, B., and Vignais, P.M., 1988, Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus, Molecular & General Genetics, 214:97–107.
Li, C., Peck Jr., H.D., LeGall, J., and Przybyla, A.E., 1987, Cloning, characterization, and sequencing of the genes encoding the large and small subunits of the periplasmic [NiFe]hydrogenase of Desulfovibrio gigas, DNA, 6:539–551.
Mulligan, M.E., and Haselkorn, R., 1989, Nitrogen-fixation (nif) genes of the cyanobacterium Anabaena sp. strain PCC 7120: the nifB-fdxN-nifS-nifU operon, J. Biol. Chem., 264:19200–19207.
Pargellis, C.A., Nunes-Duby, S.E., de Vargas, L.M., and Landy, A., 1988, Suicide recombination substrates yield covalent lambda integrase-DNA complexes and lead to identification of the active site tyrosine, J. Biol. Chem., 263:7678–7685.
Parsons, R.L., Prasad, P.V., Harshey, R.M., and Jayaram, M., 1988, Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination, Mol. Cell. Biol., 8:3303–3310.
Przybyla, A.E., Robbins, J., Menon, N., and Peck Jr., H.D., 1992, Structure-function relationships among the nickel-containing hydrogenases, FEMS Microbiol. Rev., 8:109–135.
Rousset, M., Dermoun, Z., Hatchikian, C.E., and Belaich, J.P.P., 1990, Cloning and sequencing of the locus encoding the large and small subunit genes of the periplasmic [NiFe] hydrogenase from Desulfovibrio fructosovorans, Gene, 94:95–101.
Serebriakova, L., Zorin, N.A., and Lindblad, P., 1994, Reversible hydrogenase in Anabaena variabilis ATCC 29413: presence and localization in non-N2 fixing cells, Arch. Microbiol., 161:140–144.
Wolk, C.P., 1996, Heterocyst formation, Annu. Rev. Genet., 30:59–79.
Wolk, C.P., Ernst, A., and Elhai, J., 1994, Heterocyst metabolism and development, in The Molecular Biology of Cyanobacteria, Bryant, D.A. (ed.), Kluwer Academic Publishers, Dordrecht, pp. 769–823.
Wu, L.F., and Mandrand, M.A., 1993, Microbial hydrogenases: primary structure, classification, signatures, and phylogeny, FEMS Microbiol. Rev., 10:243–269.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
Carrasco, C.D., Garcia, J.S., Golden, J.W. (1998). Programmed DNA Rearrangement of A Hydrogenase Gene During Anabaena Heterocyst Development. In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_27
Download citation
DOI: https://doi.org/10.1007/978-0-585-35132-2_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46057-9
Online ISBN: 978-0-585-35132-2
eBook Packages: Springer Book Archive