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Looking at Cells

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Part of the book series: Introductory Cell and Molecular Biology Techniques ((ICMB))

Abstract

Some of the most powerful methods of gathering data in the cell culture laboratory use visual observation. While it can take a good deal of time to develop techniques to transfer the visual observations into quantitative data, these numbers frequently still do not convey the qualitative information conveyed by visual observation. Visual observation thus remains an invaluable tool of the experienced cell culturist and cell biologist in gathering information on the status of cultures and experiments. This information is extremely valuable in making the ongoing decisions involved in establishing cell lines, maintaining healthy cultures in the laboratory, and deciding which lines of research to pursue. A trained eye and the knowledge base to interpret the observed phenomenon are therefore assets to any cell biologist.

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References

  • Boyde, A., 1985, Stereoscopic images in confocal (tandem scanning) microscopy, Science 230:1270–1272.

    Article  PubMed  CAS  Google Scholar 

  • Boyde, A., 1986, Applications of tandem scanning reflected light microscopy and three-dimensional imaging, Ann. NY Acad. Sci. 483:428–439.

    Article  PubMed  CAS  Google Scholar 

  • Chroma, T. C., 1994, Chroma Handbook of Optical Filters for Fluorescence Microscopy

    Google Scholar 

  • Eidelman, O., and Cabantchik, Z. I., 1989, Continuous monitoring of transport by fluorescence on cells and vesicles, Biochim. Biophys. Acta 988:319–334.

    PubMed  CAS  Google Scholar 

  • Fawcett, D., 1981, The Cell, 2nd ed. Philadelphia: W. B. Saunders.

    Google Scholar 

  • Fink, R., 1991, A Dozen Eggs: Time Lapse Microscopy of Normal Development, Sunderland, MA: Sinauer Associates.

    Google Scholar 

  • Fink, R., 1995, CELLabration, Sunderland, MA: Sinauer Associates.

    Google Scholar 

  • Horan, P. K., and Slezak, S. E., 1989, Stable cell membrane labelling, Nature 340:167–168.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, L. V., Walsh, M. L., and Chen, L. B., 1980, Localization of mitochondria in living cells with rhodamine 123, Proc. Natl. Acad. Sci. USA 77:990–994.

    Article  PubMed  CAS  Google Scholar 

  • Kuffler, D. P., 1990, Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs, J. Comp. Neurol. 302:720–738.

    Article  Google Scholar 

  • Pickett-Heaps, J., and Pickett-Heaps, J., 1996, Living Cells: Structure and Diversity, Sunderland, MA: Sinauer Associates.

    Google Scholar 

  • Robertson, E. A., and Schaltze, R. L., 1970, The impurities in commercial glutaraldehyde and their effect on the fixation of brain, J. Ultrastr. Res. 30:275–287.

    Article  CAS  Google Scholar 

  • Serbedzija, D. N., Frazer, S. E., and Bonner-Fraser, M., 1990, Pathways of trunk crest cell migration in the mouse embryos as revealed by vital dye labeling, Development 108:605–612.

    PubMed  CAS  Google Scholar 

  • Strieker, S. A., Paddock, S., and Schatten, G., 1990, Laser scanning confocal microscopy of living sea urchin embryos: 3-D reconstruction and calcium ion imaging, in: The American Society for Cell Biology Thirtieth Annual Meeting, Vol. III (T. Wilson, ed.), The Rockefeller University Press, San Diego, CA, pp. 113a.

    Google Scholar 

  • Wilson, T. (ed.), 1990, Confocal Microscopy, Academic Press, New York.

    Google Scholar 

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© 1998 Plenum Press, New York

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(1998). Looking at Cells. In: Introduction to Cell and Tissue Culture. Introductory Cell and Molecular Biology Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-27571-0_6

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  • DOI: https://doi.org/10.1007/978-0-585-27571-0_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-45859-0

  • Online ISBN: 978-0-585-27571-0

  • eBook Packages: Springer Book Archive

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