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Collagen I-Coated Titanium Surfaces for Bone Implantation

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Biological Interactions on Materials Surfaces

Abstract

Biological interactions at the tissue/implant material interface can be modulated by surface-linked cell-signalling biological molecules. Collagen type I, the main extracellular matrix protein of bone tissue, has been widely investigated in biomolecular surface modification of bone-contacting titanium implant devices. Literature reports on the biological effects of collagen-based coatings are, however, often contradictory. From a biomolecular surface-engineering perspective, a possible explanation is that the definition “collagen-coated surface” encompasses widely different molecular and supramolecular structures: adsorbed collagen, covalently linked collagen, crosslinked collagen, fibrillar versus monomeric collagen, and many other variation of this theme. Relevant details are not always described and proper surface characterization is often lacking. This chapter attempts to build up a rational frame of reference to describe surface modification of implant devices by collagen type I from a surface chemistry point of view, as well as to discuss relevant implications for process design.

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Abbreviations

AFM:

atomic force microscopy

Arg–Gly–Asp:

arginine –glycine –aspartic acid

BMTiS:

biochemical modification of titanium surfaces

Co:

cobalt

DAE:

double acid etched

ECM:

extracellular matrix

EDC:

1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide

NHS:

N-hydroxysuccinimide

OC:

osteocalcin

OP:

osteopontin

PBS:

phosphate-buffered saline

PDGF:

platelet-derived growth factor

PDL:

periodontal ligament

PEG:

poly(ethylene glycol)

RGD:

arginine–glycine–aspartic acid

RT-PCR:

real-time polymerase chain reaction

Ti:

titanium

Ti6Al4V:

titanium/aluminum/vanadium alloy

ToF-SIMS:

time-of-flight static secondary ion mass spectroscopy

UHV:

ultra-high vacuum

XPS:

X-ray photoelectron spectroscopy

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Acknowledgments

Most of this work was performed under the program “Coating bioattivi per dispositivi a contatto con osso” Legge 598/94 art. 11 – Ricerca, Regione Piemonte.

The significant contribution of Dr. Eng. Ilaria Cannella to the analytical work (which was part of her thesis work) is acknowledged.

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  1. Nobil Bio Ricerche s.r.l, V. Valcastellana 26, 14037, Portacomaro, AT, Italy

    Marco Morra, Clara Cassinelli, Giovanna Cascardo & Daniele Bollati

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  1. Marco Morra
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    Morra, M., Cassinelli, C., Cascardo, G., Bollati, D. (2009). Collagen I-Coated Titanium Surfaces for Bone Implantation. In: Puleo, D., Bizios, R. (eds) Biological Interactions on Materials Surfaces. Springer, New York, NY. https://doi.org/10.1007/978-0-387-98161-1_19

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