Isolated Heart Preparations, Problems, and Pitfalls

  • David R. Gross


Isolated heart preparations allow many biochemical, physiological, morphological, and pharmacological indices to be evaluated without the confounding effects of other organs, the systemic circulation, and a host of potential confounding responses such as circulating neurohumeral substances. Researchers were able to independently study the role of temperature, oxygen, calcium ions, electrical activity, and autonomic nervous system effects and, more recently, the role of specific genetic variables. When hearts of small laboratory animals are used, the preparations are highly reproducible and can be used quickly in large numbers at relatively low cost. Historically research conducted on isolated hearts led to the discovery of chemical transmission of vagal stimulation, and the importance of preload and afterload to cardiac function. Other important advances include significant understanding of ischemia-reperfusion injury, cell-based therapy, and heart preservation.


Perfusion Solution Left Ventricular Develop Pressure Heart Preparation Coronary Vasculature Retrograde Perfusion 
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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • David R. Gross
    • 1
  1. 1.Department of Veterinary BiosciencesUniversity of Illinois, Urbana Champaign College of Veterinary MedicineUrbanaUSA

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