Abstract
The free-living nematode Caenorhabditis elegans (C. elegans) is a well established biological model. Chemotaxis is an important behavior in enabling it to locate food sources such as E.coli. This study evaluates the chemotaxis of C.elegans to food following exposure to Moxidectin (Cydectin), Black Seed Oil, non-fat milk, or colostrum. A ring of bacteria (food) on nematode growth agar medium served as attractive signals to encourage C. elegans to move. Nematodes were placed in the center of the agar plate in Moxidectin, Black seed oil, colostrum or non-fat milk. Over the three day life cycle the reproduction (number of worms), movement (yes or no) and chemotaxis (number migrating to the ring of bacteria through the chemical) was recorded. Exposure to different chemicals differentially affected the total number of worms, movement and chemotaxis. Moxidectin and black seed oil inhibited migration and reproduction at the concentrations tested. Both colostrum and non-fat milk increased migration and reproduction. Caenorhabditis elegans is a sensitive multi-cellular bio and chemosensor. The bacterial ring assay is a very useful in vitro approach for evaluation of the effect of chemical exposure on chemotactic behavior and reproduction. Thus, this model can be used to better understand the molecular basis of the response to environmental exposure and for identifying molecular targets or biochemical pathways involved.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ali, B.H. and G. Blunden. 2003. “Pharmacological and toxicological properties of Nigella sativa”, Phytotherapy Research 17, 299–305.
American Society for Testing and Materials. 2002. “Standard guide for conducting laboratory soil toxicity tests with the nematode Caenorhabditis elegans”. In: Annual book of ASTM standards. Philadelphia, PA, 11.05, 1606–1616.
Bargmann C.I. and I. Mori. 1997. “Chemotaxis and thermotaxis”. In: D.L. Riddle, T. Blumenthal, B.J. Meyer, and J.R. Priess (eds.), C. elegans II, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 717–737.
Boyd, W. A., G.L. Anderson, D.B. Dusenbery, and P.L. Williams. 1999. “Computer tracking method for assessing behavioral changes in the nematode Caenorhabditis elegans" In: F.T. Price, K.V. Brix, N.K. Lane (eds.), Environmental Toxicology and Risk Assessment: Recent Achievements in Environmental Fate and Transport, vol. 9, ASTM STP 1381, American Society for Testing and Materials, West Conshohocken, PA, 2000, pp. 225–238.
Boyd, W.A. and P.L. Williams. 2003. “Comparison of the sensitivity of three nematode species to copper and their utility in aquatic and soil toxicity tests”: Environmental Toxicology Chemistry 22:2768–2774.
Brenner, S.J. 1974. ‘The genetics of Caenorhabditis elegans”. Genetics, 77, 71–94.
Clegg, E.D., H.F. LaPenotiere, D.Y., French, and M. Szilagyi. 2002. “Use of CeHR axenic medium for exposure and gene expression studies”. Reproductive Hazards Laboratory, U.S. Army Center for Environmental Health Research, Fort Detrick, MD. https://drum.umd.edu/dspace/bitstream/1903/2204/1/CeHR_medium.htm.
Condor G.A. and W.C. Campbell. 1995. “Chemotherapy of nematode infections of veterinary importance, with special reference to drug resistance”. Advances in Parasitology 35:1–84.
Cook, A., N. Aptel, V. Portillo, E. Siney, R. Sihota, L. Holden-Dye, and A. Wolstenholme. 2006. “Caenorhabditis elegans ivermectin receptors regulate locomotor behaviour and are functional orthologues of Haemonchus contortus receptors”. Molecular and Biochemical Parasitology, 147(1):118–125.
Graves, A.L., W.A. Boyd, and P.L. Williams. 2005. “Using transgenic Caenorhabditis elegans in soil toxicity testing”. Archives of Environmental Contamination Toxicology 48(4):490–4.
Davidson A. 1999. Oxford Companion to Food, “Beestings", p. 69.
Davies, A.G., and S.L. McIntire. 2004. “Using C. elegans to screen for targets of ethanol and behavior-altering drugs”. Biol Proced Online 6:113–119.
Gilleard, J.S. 2004. “The use of Caenorhabditis elegans in parasitic nematode Research”. Parasitology. 128:S49–S70 Cambridge University Press Copyrigh. Cambridge University Press.
Graves, A.L., W.A. Boyd, and P.L. Williams. 2005. “Using Transgenic Caenorhabditis elegans in Soil Toxicity Testing”. Archives of Environmental Contamination and Toxicology. Archives of Environmental Contamination Toxicology 48:490–494.
Link, E.M., Hardiman, G., Sluder, A.E., Johnson, C.D., and Liu, L.X. 2000. “Therapeutic target discovery using Caenorhabditis elegans. Pharmacogenomics” The total numbers of nematodes counted on the third day of the life cycle is presented in Table 2, 1:203–217.
Hope, I.A. 1999. “C. elegans”. Oxford University Press, Oxford.
Riddle, D.L., T. Blumenthal, B.J. Meyer, and J.R. Priess, (eds.). 1997. “C. Elegans II.” Cold Spring Harbor Laboratory Press, Plainview, New York
Tominaga, N., M. Tomoeda, S. Kohra, Y. Takao, M. Nagae, K. Ueda, Y. Ishibashi, T. Kai, and K. Arizono. 2002. “A convenient sublethal assay of alkylphenol and organotin compounds using the nematode Caenorhabditis elegans.” Journal of Health Science 48:555–559.35.
Wolstneholme, A.J. and A.T. Rogers. 2005. “Glutamate-gated chloride channels and the mode of action of the avermectin/milbemycin anthelmintics”. Parasitology. 131 Suppl:S85–95.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science+Business Media, LLC
About this paper
Cite this paper
Worku, M., Gerald, C. (2009). C. Elegans Chemotaxis and Reproduction Following Environmental Exposure. In: Nzewi, E., et al. Proceedings of the 2007 National Conference on Environmental Science and Technology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-88483-7_39
Download citation
DOI: https://doi.org/10.1007/978-0-387-88483-7_39
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-88482-0
Online ISBN: 978-0-387-88483-7
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)