Advertisement

Polymorphism of Microsatellite Sequence within ABC Transporter Genes in Phytopathogenic Fungus, Magnaporthe Grisea

  • Lin Liu
  • Chengyun Li
  • Jing Yang
  • Jinbin Li
  • Yuan Su
  • Yunyue Wang
  • Yong Xie
  • Youyong Zhu
Part of the The International Federation for Information Processing book series (IFIPAICT, volume 258)

Thirteen polyporphic microsatellite markers suitable for population genetic structure analysis and ABC transporter and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations and rice varieties. Preliminary results indicated that each locus resolved multiple alleles ranging from two to ten. There results showed that these SSR-containing genes are also polymorphic in the natural population.

Keywords

Magnaporthe grisea ABC transporter microsatellite 

References

  1. Benson G. 1999, Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27, 573-580.CrossRefPubMedPubMedCentralGoogle Scholar
  2. Cecconi F., Alvarez-Bolado G., Meyer B.I., Roth K.A., Gruss P. 1998, Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development, Cell 94, 727-737.CrossRefPubMedGoogle Scholar
  3. Dean Ralph A., Talbot Nlcholas J., Ebbole Danlel J. Ebbole, Mark L. Farman, Thomas K. Mltchell, Marc J. Orbach, Mlchael Thon, Resham Kulkarnl, Jin-Rong Xu, Huaqln Pan, Nlck D. Read, Yong-Hwan Lee, lgnazlo Carbone, Doug Brown, Yeon Yee Oh, Nlcole Donofrlo, Jun Seop Jeong, Darren M. Soanes, Slavlca Djonovlc, Elena Kolomlets, Cathryn Rehmeyer, Welxi Li, Michael Hardlng, Soonok Klm, Marc-Henrl Lebrun, Heidl Bohnert, Sean Coughlan, Jonathan Butler, Sarah Calvo, Li-Jun Ma, Robert Nicol, Seth Purcell, Chad Nusbaum, James E. Galagan & Bruce W. Blrren 2005, The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434, 980-986.CrossRefPubMedGoogle Scholar
  4. Edward M. Marcotte, Matteo pellegrini, Todd O. Yeates and David Eisenberg 1998, A census of protein repeats, J. Mol. Biol. 293, 151-160.CrossRefGoogle Scholar
  5. Higgins C.F. 1992, ABC transporters: from microorganisms to man. Annu. Rev. Cell. Biol. 8, 67-113.CrossRefPubMedGoogle Scholar
  6. Keleher C.A., Redd M.J., Schultz J., Carlson M., Johnson A.D. 1992, Ssn6-Tup1 is a general repressor of transcription in yeast, Cell 68, 709-719.CrossRefPubMedGoogle Scholar
  7. Li Cheng-yun, Li Jinbin, Zhou Xiao-gang, Zhang Shao-song, Dong Ai-rong, Xu Ming-hui 2005, Frequency and Distribution of Microsatellite sequence in Open Reading Frames of Rice Blast Fungus, Magnaporthe grisea. Chinese J. Rice Science 19(2), 167-173, 2005. (in Chinese with English abstract).Google Scholar
  8. Michaelis S., Berkower C. 1995, Sequence comparison of yeast ATP-binding cassette proteins. Cold Spring Harbor Symp Quant Biol 60, 291-307.CrossRefPubMedGoogle Scholar
  9. Ou S. H. Rice diseases 1985, 2nd Edition. Commonwealth Mycological Institute, Kew, UK, 1985. 380.Google Scholar
  10. Rozen S., Skaletsky H. 2000, Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 132, 365-386.PubMedGoogle Scholar
  11. Saxena K., Gaitatzes, C., Walsh, M.T., Eck, M., Neer, E.J., and Smith, T.F. 1996, Analysis of the physical properties and molecular modeling of Sec13: a WD repeat protein involved in vesicular traffic, Biochemistry 35, 15215-15221.CrossRefPubMedGoogle Scholar
  12. Stifani S., Blaumueller C.M., Redhead N.J., Hill R.E., Artavanis Tsakonas S. 1992, Human homologs of a Drosophila Enhancer of split gene product define a novel family of nuclear proteins [published erratum appears in Nat. Genet. Dec2(4), 119-127.Google Scholar
  13. Sweigard, J.A., Orbach, M.J., Valent, B., Chumley, F.G. 1990, A miniprep procedure for isolating genomic DAN from Magnaporthe grisea, Fungal. Genet. Newslett 37, 4Google Scholar
  14. Vaisman N., Tsouladze A., Robzyk K., Ben-Yehuda S., Kupiec M., Kassir Y. 1995 The role of Saccharomyces cerevisiae Cdc40p in DNA replication and mitotic spindle formation and/or maintenance, Mol. Gen. Genet 247, 123-136.CrossRefPubMedGoogle Scholar
  15. Young E.T., Sloan, J.S. and Riper K.V. 2000, Trinucleotide repeats are clustered in regulatory genes in Saccharomyces cerevisiae. Genetics 154, 1053-1068.PubMedPubMedCentralGoogle Scholar
  16. Zeigler, R. S., Scott, R.P., Leung, H., Bordeos, A. A., Kumar, J., and Nelson, R. J. 1997, Evidence of parasexual exchange of DNA in the rice blast fungus challenges its exclusive clonality. Phytopathology 87, 284-294.CrossRefPubMedGoogle Scholar

Copyright information

© IFIP International Federation for Information Processing 2008

Authors and Affiliations

  • Lin Liu
    • 1
  • Chengyun Li
    • 1
  • Jing Yang
    • 1
  • Jinbin Li
    • 2
  • Yuan Su
    • 1
  • Yunyue Wang
    • 1
  • Yong Xie
    • 1
  • Youyong Zhu
    • 1
  1. 1.Plant Protection CollegeYunnan Agricultural UniversityChina
  2. 2.Plant Protection Research Institute of Yunnan Academy of Agricultural SciencesKunmingChina

Personalised recommendations