Advertisement

Differentially Gene Expression in the Brain of Common Carp (Cyprinus carpio) Response to Cold Acclimation

  • Liqun Liang
  • Shaowu Li
  • Yumei Chang
  • Yong Li
  • Xiaowen Sun
  • Qingquan Lei
Part of the The International Federation for Information Processing book series (IFIPAICT, volume 258)

There are a variety of approaches to identify groups of genes that change in expression in response to a particular stimulus or environment. We here describe the application of suppression subtractive hybridization (SSH) for isolation and identification genes in the brain of common carp (Cyprinus carpio) under cold temperatures. The materials were prepared through cooling the hybrid F2 of purse red carp (cold-tolerant strains) and bighead carp (cold-sensitive species) to different regimes of temperatures. A subtracted cDNA library containing 2000 clones was constructed. About 60 positive clones were identified to express differentially by dot blotting in screening 480 clones. Sequencing 26 clones and aligning in GenBank/EMBL database using blastn searching engine, 15 genes showed higher similarities with 85-98%. These annotated genes contained (1) genes for transcription factors and gene products involved in signal transduction pathways such as zinc-finger protein, brevican; (2) genes involved in lipid metabolism such as Acyl-CoA synthetases, and (3) genes involved in the translational machinery such as cytochrome c oxidase, ependymin glycoprotein. In addition, real-time PCR was also conducted to validate these genes. To sum up, we believe this study will make an important contribution to elucidate the possible mechanisms on fish cold tolerance at a molecular level.

Keywords

cold tolerance suppression subtractive hybridization (SSH) real-time PCR Cyprinus carpio 

References

  1. Arnab M, et al. Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. PNAS, 2004, 101:6309-6314CrossRefGoogle Scholar
  2. Baldwin J, Hochachka PW. Functional significance of isoenzymes in thermal acclimatizationacetylcholinesterase fromtrout brain, Bioch J, 1970, 116:883-887CrossRefGoogle Scholar
  3. Battersby BJ, et al. Influence of acclimation temperature on mitochondrial DNA, RNA, and enzymes in skeletal muscle. Am. J. Physiol., 1998, 275:R905-R912PubMedGoogle Scholar
  4. Beattie JH, et al. Cold-induced expression of the metallothionein-1 gene in brown adipose tissue of rats. Am. J. Physoil., 1996, 270:R971-R977Google Scholar
  5. Brown WD. Glucose metabolism in carp. J. Cellular Comp. Physiol., 1960, 55:81-85CrossRefGoogle Scholar
  6. Cossins AR, et al. The role of desaturases in cold-induced lipid restructing. Biochem Soc. Trans., 2002, 30(6):1082-1086Google Scholar
  7. Fraser NHC, et al. Temperature-dependent switch between diurnal and nocturnal foraging in salmon. Proc. R. Soc., 1993, 252:135-139CrossRefGoogle Scholar
  8. Gaigg B, et al. Depletion of acyl-coenzyme A-binding protein affects sphingolipid systhesis and causes vesicle accumulation and membrane defects in Saccharonmyces cerevisiae. Mol. Biol. Cell., 2001, 12:1147-1160CrossRefPubMedPubMedCentralGoogle Scholar
  9. Gary SC, et al. BEHAB/brevican: a brain-specific lectican implicated in gliomas and glial cell motility. Curr. Opin. Neurobiol., 1998, 8(5):576-581CrossRefPubMedGoogle Scholar
  10. Hardewig I, et al. Temperature-dependent expression of cytochrome-c oxidase in Antarctic and temperate fish. Am. J. Physiol., 1999, 277:R508-R516PubMedGoogle Scholar
  11. Ju Z, et al. Differential gene expression in the brain of channel catfish (Ictalurus punctatus ) in response to cold acclimation. Mol. Genet Genomics., 2002, 268:87-95CrossRefPubMedGoogle Scholar
  12. Kloepper-Sams PJ, Stegeman JJ. Effects of temperature acclimation on the expression of hepatic cytochrome P4501A mRNA and protein in the fish Fundulus heteroclitus. Arch. Biochem. Biophys., 1992, 99:38-46CrossRefGoogle Scholar
  13. Pickett MH, et al. Seasonal variation in the level of antifreeze protein mRNA from the winter flounder. Biochim. Biophys. Acta., 1983, 739:97-104CrossRefPubMedGoogle Scholar
  14. Poli A, et al. Neurochemical changes in cerebellum of goldfish exposed to various temperatures. Neurochem. Res., 1997, 22:141-149CrossRefPubMedGoogle Scholar
  15. Rodnick KJ, Sidell BD. Cold acclimation increases carnitine palmitoyltransferase activity in oxidative muscle of striped bass. Am. J. Physiol., 1994, 266:R405-R412PubMedGoogle Scholar
  16. Roussel D, et al. Increase in the adenine nucleotide translocase content of ducking subsarcolemmal mitochondria during cold acclimation. FEBS Letters, 2000, 477:141-144CrossRefPubMedGoogle Scholar
  17. Roy R, et al. Regulation of membrane lipid bilayer structure during seasonal variation: a study on the brain membranes of Clarias batrachus. Biochim. Biophys. Acta., 1997, 1323(1):65-74CrossRefPubMedGoogle Scholar
  18. Tang SJ, et al. Cold-induced ependymin expression in zebrafish and carp brain: implications for cold acclimation. FEBS Letters, 1999, 459:95-99CrossRefPubMedGoogle Scholar
  19. Thillart V, Modderkolk J. The effect of acclimation temperature on the activation energies of state III respiration and the unsaturation of membrane lipids of gold fish mitochondria. Biochim. Biophys. Acta., 1978, 510:38-51CrossRefPubMedGoogle Scholar
  20. Tiku PE, et al. Cold-inducible expression of desaturase by transcriptional an post-translational mechanisms. Science, 1996, 271:815-818CrossRefPubMedGoogle Scholar
  21. Wodtke E. Temperature adaptation of biological membranes. Compensation of the molar activity of cytochrome c oxidase in the mitochondrial energy-transducing membrane during thermal acclimation of the carp (Cyprinus carpio L.). Biochim. Biophys. Acta., 1981, 640:710-720CrossRefPubMedGoogle Scholar
  22. Yeo YK, et al. Ether lipid composition and molecular species alterations in carp brain (Cyprinus carpio L.) during normoxic temperature acclimation. Neurochem. Res., 1997, 22 (10):57-64CrossRefGoogle Scholar

Copyright information

© IFIP International Federation for Information Processing 2008

Authors and Affiliations

  • Liqun Liang
    • 1
  • Shaowu Li
    • 1
  • Yumei Chang
    • 1
  • Yong Li
    • 1
  • Xiaowen Sun
    • 1
  • Qingquan Lei
    • 2
  1. 1.Chinese Academy of Fishery SciencesHeilongjiang River Fisheries Research InstituteChina
  2. 2.Harbin University of Science and TechnologyChina

Personalised recommendations