Abstract
A novel evanescent-based biosensor (Endotect™, ThreeFold Sensors, Inc.) was developed with laser-based fiber optics using fluorescent dyelabeled recombinant human estrogen receptor-α (rhERα) and hERβ as probes. A three-tiered approach evaluating various steps in the formation of the estrogen- receptor complex and its subsequent activity was developed for instrument calibration to detect estrogen mimics in biological samples, water and soil. Using this approach, binding affinities and activities of certain known estrogen mimics were determined for their use as calibrator molecules. Results indicated rhERα and rhERβ may be employed as probes to distinguish estrogen mimics with a broad range of affinities. In addition, application of the biosensor for detecting DNA-binding proteins in human tissue extracts was demonstrated. The later studies suggest the biosensor may be used as a clinical laboratory tool for assessing tumor marker proteins.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
ICCVAM/NICEATM Final Report, Expert Panel Evaluation of the Validation Status of in vitro Test Methods for Detecting Endocrine Disruptors: Estrogen Receptor and Androgen Receptor Binding and Transcriptional Activation Assays, 2002 (http:// iccvam.niehs.nih.gov).http://iccvam.niehs.nih.gov).
J. L. Wittliff, R. Pasic, K. I. Bland: Steroid and Peptide Hormone Receptors: Methods, Quality Control and Clinical Use, in Bland KI, Copeland III EM (eds): The Breast: Comprehensive Management of Benign and Malignant Diseases. Philadelphia, PA, W. B. Saunders Co, 458–498, 1998.
R. H. Smith, W. J. Lemon, J. L. Erb, J. R. Erb-Downward, J. G. Downward, O. E. Ulrich, and J. L. Wittliff, Development of Kinetic Ligand-binding Assays Using a Fiber Optic Sensor, Clin. Chem. 45(9), 1683–1685 (1999).
E. A. E. Garber, J. L. Erb, J. G. Downward, E. M. Priuska, J. L. Wittliff, W. Feng, J. Magner, and G. L. Larsen, Biosensor, ELISA, and Frog Embryo Teratogenesis Assay: Xenopus (FETAX) Analysis of Water Associated with Frog Malformations in Minnesota, Proc. Soc. Photo-Optical Instrumentation Engineers (SPIE) 4206, 147–158 (2001).
J. L. Erb, E. A. E. Garber, J. G. Downward IV, and E. M. Priuska, Data from an Estrogen Receptor-based Biosensor Correlates with Evidence of Frog Malformation and Demonstrates a Differential Response of hERα & β to Beneficial and Harmful Estrogenic Compounds, In: Proc. 2nd Intl. Conf. Pharmaceuticas & Endocrine Disrupting Chemicals in Water, p. 203–217, Westerville, OH, The National Ground Water Association (2001).
T. E. Hirchfield, Fluorescent Immunoassay Employing Optical Fiber in a Capillary Tube, U.S. Patent No. 4,447,546 (1984).
W. Raffelsberger and J. L. Wittliff, A Novel Approach for Comparing Ligand Binding Results from Titration and Competition Analyses to Study Hormone Mimics, J. Clin. Ligand Assay 20(4), 275–280 (1997).
J. L. Wittliff, L. L. Wenz, J. Dong, Z. Nawaz, and T. R. Butt, Expression and Characterization of an Active Estrogen Receptor as a Ubiquitin Fusion Protein from Escherichia coli, J. Biol. Chem. 265(35), 22016–22025 (1990).
K. Graumann, J. L. Wittliff, W. Raffelsberger, L. Miles, A. Jungbauer, and T.R. Butt, Structural and Functional Analysis of N-terminal Point Mutants of the Human Estrogen Receptor, J. Steroid Biochem. Mol. Biol. 57(5–6), 292–300 (1996).
C. R. Lyttle, P. Damian-Matsumura, H. Juul, and T. R. Butt, Human Estrogen Receptor Regulation in a Yeast Model System and Studies on Receptor Agonists and Antagonists, J. Steroid Biochem. Mol. Biol. 42(7), 77–685 (1992).
J. L. Wittliff, P. Folk, J. Dong, C. Schaupp, and T. R. Butt, Characteristics of the Human Estrogen Receptor Protein Produced in Microbial Expression Systems. In: V. K. Moudgil (ed.), Steroid Hormone Receptors: Basic and Clinical Aspects, pp. 473–501, Birkhauser Boston (1993).
T. L. Kruer, I. A. Smolenkova and J. L. Wittliff, Expression of Novel ERE-Binding Proteins in Breast and Uterine Cells, American Society for Biochemistry & Molecular Biology, Abstract # 1381 (2006).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer Science+Business Media, LLC
About this paper
Cite this paper
Wittliff, J.L., Andres, S.A., Kruer, T.L., Kerr, D.A., Smolenkova, I.A., Erb, J.L. (2008). Biosensors for Detecting Estrogen-like Molecules and Protein Biomarkers. In: Kang, K.A., Harrison, D.K., Bruley, D.F. (eds) Oxygen Transport to Tissue XXIX. Advances In Experimental Medicine And Biology, vol 614. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-74911-2_35
Download citation
DOI: https://doi.org/10.1007/978-0-387-74911-2_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-74910-5
Online ISBN: 978-0-387-74911-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)