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Combinatorially Developed Peptide Receptors for Biosensors

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Combinatorial Methods for Chemical and Biological Sensors

Part of the book series: Integrated Analytical Systems ((ANASYS))

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Abstract

Various combinatorial libraries were screened for short peptides of 4–10 mer, which were used as sensor molecules for capturing target chemicals or biomolecules. Immuno-antibodies can be synthesized in the living bodies of higher animals even for low-molecular-weight nonnatural chemical compounds, such as dioxins or PCBs. Recently, some peptide ligands that can even bind to inorganic crystals have been reported. This indicates that the 20 natural amino acids have the potential to recognize almost all types of molecules and substances. The question arises whether one should design a “rational” mini library of peptides consisting of a limited number of amino acids according to the motifs in epitopes or paratopes or the binding pocket sequences in receptors, or a completely “random” combinatorial library containing all sequences. If one wants to obtain a peptide binder to target a small chemical compound, the answer is a “random” library, since the molecular interaction between the target compound and an amino acid cannot be precisely predicted beforehand. In this section, we discuss the possibility of using short combinatorial peptides as binders for biosensors to detect chemical compounds.

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Authors and Affiliations

  1. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, 305-8566, Ibaraki, Japan

    Chikashi Nakamura & Jun Miyake

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  1. Chikashi Nakamura
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  2. Jun Miyake
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Correspondence to Chikashi Nakamura .

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Editors and Affiliations

  1. Global Research Center, General Electric Company, 12309, Niskayuna, NY, USA

    Radislav A. Potyrailo

  2. Nanobiotechnology, Lausitz University of Applied Sciences, 01968, Senftenberg, Germany

    Vladimir M. Mirsky

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Nakamura, C., Miyake, J. (2009). Combinatorially Developed Peptide Receptors for Biosensors. In: Potyrailo, R.A., Mirsky, V.M. (eds) Combinatorial Methods for Chemical and Biological Sensors. Integrated Analytical Systems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-73713-3_8

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