Diversity-Oriented Fluorescence Library Approach for Novel Sensor Development
- 928 Downloads
Fluorescence-based sensors are of extreme importance and have found applications in various fields. Although the demand for useful fluorescence sensors is acute, the conventional target-oriented approach has limited the scope and speed of novel sensor discovery. To make a breakthrough in this field, we introduced a new diversity-oriented fluorescence library approach (DOFLA) using combinatorial fluorescence compound libraries with huge structural diversity. Surprisingly, specific and unique sensors for a broad range of analytes from macromolecules including DNA, RNA, and proteins to signaling small molecules such as GTP, glutathione could be discovered from the library pools, demonstrating the universal applicability of this approach.
KeywordsCombinatorial Chemistry Fluorescence Sensor Pyridinium Salt Benzimidazolium Ring Sensor Discovery
The research and discoveries summarized herein were mainly based on the continuous contributions from the previous and present Chang group members. We are very grateful to all the individuals involved with these projects, with particular appreciations to: Dr. Gustavo R. Rosania, Dr. Shao Q. Yao, Dr. Jaeki Min, Dr. Jaewook Lee, Dr. Qian Li, Dr. Young-Hoon Ahn, Mr. Jun-Seok Lee, and Ms. Yunkyung Kim.
- 1.Valeur, B. Molecular fluorescence: Principles and applications; Wiley: Weinheim, New York, 2002Google Scholar
- 2.Lakowicz, J. R. Principles of fluorescence spectroscopy; 2nd ed.; Kluwer/Plenum: New York, 1999Google Scholar
- 16.Srinivasan, N.; Kilburn, J. D., Combinatorial approaches to synthetic receptors, Curr Opin Chem Biol 2004, 8, 305–310Google Scholar
- 25.Sudlow, G.; Birkett, D. J.; Wade, D. N., Characterization of 2 specific drug binding-sites on human-serum albumin, Mol Pharmacol 1975, 11, 824–832Google Scholar
- 26.Sudlow, G.; Birkett, D. J.; Wade, D. N., Further characterization of specific drug binding-sites on human-serum albumin, Mol Pharmacol 1976, 12, 1052–1061Google Scholar
- 27.Irikura, M.; Takadate, A.; Goya, S.; Otagiri, M., 7-alkylaminocoumarin-4-acetic acids as fluorescent-probe for studies of drug-binding sites on human serum-albumin, Chem Pharm Bull 1991, 39, 724–728Google Scholar
- 30.Lee, J. W.; Jung, M.; Rosania, G. R.; Chang, Y. T., Development of novel cell-permeable DNA sensitive dyes using combinatorial synthesis and cell-based screening, Chem Comm 2003, 1852–1853Google Scholar
- 36.Burma, D. P., GTP acts as the master key in regulating diverse physiological processes, J Sci Ind Res 1988, 47, 65–80Google Scholar
- 37.Pogson, C. I., Guanine nucleotides and their significance in biochemical processes, Am J Clin Nutr 1974, 27, 380–402Google Scholar
- 39.Deneke, S. M.; Fanburg, B. L., Regulation of cellular glutathione, Am J Physiol 1989, 257, L163–L173Google Scholar