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Functional Nucleic Acids for Analytical Applications pp 255–270Cite as

Aptamers in Affinity Separations:Capillary Electrophoresis

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Part of the book series: Integrated Analytical Systems ((ANASYS))

Abstract

Assays employing aptamers in capillary electrophoresis (CE), including competitive and noncompetitive assays, fluorescence polarization (FP) assays, nonequilibrium capillary electrophoresis of equilibrium mixtures, and affinity-polymerase chain reaction-CE assays, are summarized. These assays can be used to estimate dissociation rate and equilibrium binding constants, determine binding stoichiometries, study molecular interactions, and quantitatively determine specific analytes (e.g., proteins) in complex media. They can potentially be completed in under 60 s, detect zeptomol (10−24) amounts of analyte, be utilized in complex media with little or no cross reaction, and target a number of different analytes of biological, environmental, and clinical importance. This chapter briefly overviews the process of aptamer selection using CE and discusses the various CE-based bioanalytical methods that have been used to study biomolecular interactions.

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Authors
  1. Jeffrey W. Guthrie
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  2. Yuanhua Shao
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  3. X. Chris Le
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Editor information

Editors and Affiliations

  1. Department of Biochemistry and Biomedical Sciences, McMaster University Hamilton, Ontario, Canada

    Li Yingfu

  2. Department of Chemistry, University of Illinois at Urbana-Champaign Urbana, Illinois, USA

    Lu Yi

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Guthrie, J.W., Shao, Y., Le, X.C. (2009). Aptamers in Affinity Separations:Capillary Electrophoresis. In: Yingfu, L., Yi, L. (eds) Functional Nucleic Acids for Analytical Applications. Integrated Analytical Systems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-73711-9_9

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