Skip to main content
SpringerLink
Log in

The Dot/Icm Type IVB Secretion System

  • Chapter
Legionella pneumophila

Part of the book series: Infectious Diseases And Pathogenesis ((IAPA))

  • 1378 Accesses

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Amor, J. C., J. Swails, X. Zhu, C. R. Roy, H. Nagai, A. Ingmundson, X. Cheng, and R. A. Kahn. 2005. The structure of RalF, an ADP-ribosylation factor guanine nucleotide exchange factor from Legionella pneumophila, reveals the presence of a cap over the active site. J Biol Chem 280:1392–400.

    Article  PubMed  CAS  Google Scholar 

  • Backert, S. and T. F. Meyer. 2006. Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol 9:207–17.

    Article  PubMed  CAS  Google Scholar 

  • Bar-Nun, S. 2005. The role of p97/Cdc48p in endoplasmic reticulum-associated degrada tion: from the immune system to yeast. Curr Top Microbiol Immunol 300:95–125.

    Article  PubMed  CAS  Google Scholar 

  • Bardill, J. P., J. L. Miller, and J. P. Vogel. 2005. IcmS-dependent translocation of SdeA into macrophages by the Legionella pneumophila type IV secretion system. Mol Microbiol 56:90–103.

    Article  PubMed  CAS  Google Scholar 

  • Berger, K. H. and R. R. Isberg. 1993. Two distinct defects in intracellular growth comple mented by a single genetic locus in Legionella pneumophila. Mol Microbiol 7:7–19.

    Article  PubMed  CAS  Google Scholar 

  • Berk, S. G., R. S. Ting, G. W. Turner, and R. J. Ashburn. 1998. Production of respirable vesicles containing live Legionella pneumophila cells by two Acanthamoeba spp. Appl Environ Microbiol 64:279–86.

    PubMed  CAS  Google Scholar 

  • Boschiroli, M. L., Ouahrani-Bettache, S., Foulongne, V., Michaux-Charachon, S., Bourg, G., Allardet-Servent, A., Cazevieille, C., Lavigne, J. P., Liautard, J. P., Ramuz, M., and D. O’Callaghan. 2002. Type IV secretion and Brucella virulence. Vet Microbiol 90:341–8.

    Article  PubMed  CAS  Google Scholar 

  • Bruggemann, H., A. Hagman, M. Jules, O. Sismeiro, M. A. Dillies, C. Gouyette, F. Kunst, M. Steinert, K. Heuner, J. Y. Coppee, and C. Buchrieser. 2006. Virulence strategies for infecting phagocytes deduced from the in vivo transcriptional program of Legionella pneumophila. Cell Microbiol 8:1228–40.

    Article  PubMed  CAS  Google Scholar 

  • Buscher, B. A., G. M. Conover, J. L. Miller, S. A. Vogel, S. N. Meyers, R. R. Isberg, and J. P. Vogel. 2005. The DotL protein, a member of the TraG-coupling protein family, is essential for viability of Legionella pneumophila strain Lp02. J Bacteriol 187:2927–38.

    Article  PubMed  CAS  Google Scholar 

  • Campodonico, E. M., L. Chesnel, and C. R. Roy. 2005. A yeast genetic system for the identification and characterization of substrate proteins transferred into host cells by the Legionella pneumophila Dot/Icm system. Mol Microbiol 56:918–33.

    Article  PubMed  CAS  Google Scholar 

  • Celli, J. and J. P. Gorvel. 2004. Organelle robbery: Brucella interactions with the endoplasmic reticulum. Curr Opin Microbiol 7:93–7.

    Article  PubMed  CAS  Google Scholar 

  • Chen, J., K. S. de Felipe, M. Clarke, H. Lu, O. R. Anderson, G. Segal, and H. A. Shuman. 2004. Legionella effectors that promote nonlytic release from protozoa. Science 303:1358–61.

    Article  PubMed  CAS  Google Scholar 

  • Christie, P. J. and J. P. Vogel. 2000. Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells. Trends Microbiol 8:354–60.

    Article  PubMed  CAS  Google Scholar 

  • Christie, P. J., K. Atmakuri, V. Krishnamoorthy, S. Jakubowski, and E. Cascales. 2005. Biogenesis, architecture, and function of bacterial type IV secretion systems. Annu Rev Microbiol 59:451–85.

    Article  PubMed  CAS  Google Scholar 

  • Coers, J., C. Monahan, and C. R. Roy. 1999. Modulation of phagosome biogenesis by Legionella pneumophila creates an organelle permissive for intracellular growth. Nat Cell Biol 1:451–3.

    Article  PubMed  CAS  Google Scholar 

  • Coers, J., J. C. Kagan, M. Matthews, H. Nagai, D. M. Zuckman, and C. R. Roy. 2000. Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth. Mol Microbiol 38:719–36.

    Article  PubMed  CAS  Google Scholar 

  • Conover, G. M., I. Derré, J. P. Vogel, and R. R. Isberg. 2003. The Legionella pneumophila LidA protein: a translocated substrate of the Dot/Icm system associated with maintenance of bacterial integrity. Mol Microbiol 48:305–21.

    Article  PubMed  CAS  Google Scholar 

  • Derré, I. and R. R. Isberg. 2004. Legionella pneumophila replication vacuole formation involves rapid recruitment of proteins of the early secretory system. Infect Immun 72:3048–53.

    Article  PubMed  CAS  Google Scholar 

  • Derré, I. and R. R. Isberg. 2005. LidA, a translocated substrate of the Legionella pneumophila type IV secretion system, interferes with the early secretory pathway. Infect Immun 73:4370–80.

    Article  PubMed  CAS  Google Scholar 

  • Dorer, M. S., D. Kirton, J. S. Bader, and R. R. Isberg. 2006. RNA interference analysis of Legionella in Drosophila cells: exploitation of early secretory apparatus dynamics. PLoS Pathog 2:e34.

    Article  PubMed  CAS  Google Scholar 

  • Duménil, G. and R. R. Isberg. 2001. The Legionella pneumophila IcmR protein exhibits chaperone activity for IcmQ by preventing its participation in high-molecular-weight complexes. Mol Microbiol 40:1113–27.

    Article  PubMed  Google Scholar 

  • Duménil, G., T. P. Montminy, M. Tang, and R. R. Isberg. 2004. IcmR-regulated membrane insertion and efflux by the Legionella pneumophila IcmQ protein. J Biol Chem 279:4686–95.

    Article  PubMed  Google Scholar 

  • Gal-Mor, O. and G. Segal. 2003a. Identification of CpxR as a positive regulator of icm and dot virulence genes of Legionella pneumophila. J Bacteriol 185:4908–19.

    Article  CAS  Google Scholar 

  • Gal-Mor, O. and G. Segal. 2003b. The Legionella pneumophila GacA homolog (LetA) is involved in the regulation of icm virulence genes and is required for intracellular multiplication in Acanthamoeba castellanii. Microb Pathog 34:187–94.

    Article  CAS  Google Scholar 

  • Gal-Mor, O., T. Zusman, and G. Segal. 2002. Analysis of DNA regulatory elements required for expression of the Legionella pneumophila icm and dot virulence genes. J Bacteriol 184:3823–33.

    Article  PubMed  CAS  Google Scholar 

  • Horwitz, M. A. and F. R. Maxfield. 1984. Legionella pneumophila inhibits acidification of its phagosome in human monocytes. J Cell Biol 99:1936–43.

    Article  PubMed  CAS  Google Scholar 

  • Hueck, C. J. 1998. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62:379–433.

    PubMed  CAS  Google Scholar 

  • Kagan, J. C. and C. R. Roy. 2002. Legionella phagosomes intercept vesicular traffic from endoplasmic reticulum exit sites. Nat Cell Biol 4:945–54.

    Article  PubMed  CAS  Google Scholar 

  • Kagan, J. C., M. P. Stein, M. Pypaert, and C. R. Roy. 2004. Legionella subvert the functions of Rab1 and Sec22b to create a replicative organelle. J Exp Med 199:1201–11.

    Article  PubMed  CAS  Google Scholar 

  • Kirby, J. E., J. P. Vogel, H. L. Andrews, and R. R. Isberg. 1998. Evidence for pore-forming ability by Legionella pneumophila. Mol Microbiol 27:323–36.

    Article  PubMed  CAS  Google Scholar 

  • Lu, H. and M. Clarke. 2005. Dynamic properties of Legionella-containing phagosomes in Dictyostelium amoebae. Cell Microbiol 7:995–1007.

    Article  PubMed  CAS  Google Scholar 

  • Luo, Z. Q. and R. R. Isberg. 2004. Multiple substrates of the Legionella pneumophila Dot/Icm system identified by interbacterial protein transfer. Proc Natl Acad Sci USA 101:841–6.

    Article  PubMed  CAS  Google Scholar 

  • Machner, M. P. and R. R. Isberg. 2006. Targeting of host rab GTPase function by the intravacuolar pathogen Legionella pneumophila. Dev Cell 11:47–56.

    Article  PubMed  CAS  Google Scholar 

  • Marra, A., S. J. Blander, M. A. Horwitz, and H. A. Shuman. 1992. Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages. Proc Natl Acad Sci USA 89:9607–11.

    Article  PubMed  CAS  Google Scholar 

  • Matthews, M. and C. R. Roy. (2000). Identification and subcellular localization of the Legionella pneumophila IcmX Protein: a factor essential for establishment of a replicative organelle in eukaryotic host cells. Infect Immun 68:3971–3982.

    Article  PubMed  CAS  Google Scholar 

  • Molofsky, A. B. and M. S. Swanson. (2004). Differentiate to thrive: lessons from the Legionella pneumophila life cycle. Mol. Microbiol. 53:29–40.

    Article  PubMed  CAS  Google Scholar 

  • Nagai, H. and C. R. Roy. 2003. Show me the substrates: modulation of host cell function by type IV secretion systems. Cell Microbiol 5:373–83.

    Article  PubMed  CAS  Google Scholar 

  • Nagai, H., J. C. Kagan, X. Zhu, R. A. Kahn, and C. R. Roy. 2002. A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes. Science 295:679–82.

    Article  PubMed  CAS  Google Scholar 

  • Ninio, S., D. M. Zuckman-Cholon, E. D. Cambronne, and C. R. Roy. 2005. The Legionella IcmS-IcmW protein complex is important for Dot/Icm-mediated protein translocation. Mol Microbiol 55:912–26.

    Article  PubMed  CAS  Google Scholar 

  • Rabin, S. D., J. L. Veesenmeyer, K. T. Bieging, and A. R. Hauser. 2006. A C-terminal domain targets the Pseudomonas aeruginosa cytotoxin ExoU to the plasma membrane of host cells. Infect Immun 74:2552–61.

    Article  PubMed  CAS  Google Scholar 

  • Rowbotham, T. J. 1986. Current views on the relationships between amoebae, legionellae and man. Isr J Med Sci 22:678–89.

    PubMed  CAS  Google Scholar 

  • Roy, C. R. and R. R. Isberg. 1997. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infect Immun 65:571–8.

    PubMed  CAS  Google Scholar 

  • Roy, C. R., K. H. Berger, and R. R. Isberg. 1998. Legionella pneumophila DotA protein is required for early phagosome trafficking decisions that occur within minutes of bacterial uptake. Mol Microbiol 28:663–74.

    Article  PubMed  CAS  Google Scholar 

  • Sauer, J. D., J. G. Shannon, D. Howe, S. F. Hayes, M. S. Swanson, and R. A. Heinzen. 2005. Specificity of Legionella pneumophila and Coxiella burnetii vacuoles and versatility of Legionella pneumophila revealed by coinfection. Infect Immun 73:4494–504.

    Article  PubMed  CAS  Google Scholar 

  • Segal, G. and H. A. Shuman. 1998. Intracellular multiplication and human macrophage killing by Legionella pneumophila are inhibited by conjugal components of IncQ plasmid RSF1010. Mol Microbiol 30:197–208.

    Article  PubMed  CAS  Google Scholar 

  • Segal, G., J. J. Russo, and H. A. Shuman. 1999. Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila. Mol Microbiol 34:799–809.

    Article  PubMed  CAS  Google Scholar 

  • Segal, G., M. Feldman, and T. Zusman. 2005. The Icm/Dot type-IV secretion systems of Legionella pneumophila and Coxiella burnetii. FEMS Microbiol Rev 29:65–81.

    Article  PubMed  CAS  Google Scholar 

  • Sexton, J. A. and J. P. Vogel. 2002. Type IVB secretion by intracellular pathogens. Traffic 3:178–85.

    Article  PubMed  CAS  Google Scholar 

  • Sexton, J. A., J. L. Miller, A. Yoneda, T. E. Kehl-Fie, and J. P. Vogel. 2004a. Legionella pneumophila DotU and IcmF are required for stability of the Dot/Icm complex. Infect Immun 72:5983–92.

    Article  CAS  Google Scholar 

  • Sexton, J. A., J. S. Pinkner, R. Roth, J. E. Heuser, S. J. Hultgren, and J. P. Vogel. 2004b. The Legionella pneumophila PilT homologue DotB exhibits ATPase activity that is critical for intracellular growth. J Bacteriol 186:1658–66.

    Article  CAS  Google Scholar 

  • Sexton, J. A., H. J. Yeo, and J. P. Vogel. 2005. Genetic analysis of the Legionella pneumophila DotB ATPase reveals a role in type IV secretion system protein export. Mol Microbiol 57:70–84.

    Article  PubMed  CAS  Google Scholar 

  • Shi, C., V. Forsbach-Birk, R. Marre, and T. L. McNealy. 2006. The Legionella pneumophila global regulatory protein LetA affects DotA and Mip. Int J Med Microbiol 296:15–24.

    Article  PubMed  CAS  Google Scholar 

  • Shohdy, N., J. A. Efe, S. D. Emr, and H. A. Shuman. 2005. Pathogen effector protein screening in yeast identifies Legionella factors that interfere with membrane trafficking. jt>Proc Natl Acad Sci USA 102:4866–71.

    Google Scholar 

  • Swanson MS and B.K. Hammer. 2000 Legionella Pneumophila pathogenesis: a fateful journey from amoebae to macrophages. Annu Rev Microbiol 54:567–613.

    Article  PubMed  CAS  Google Scholar 

  • Uchiya, K., M. A. Barbieri, K. Funato, A. H. Shah, P. D. Stahl, and E. A. Groisman. 1999. A Salmonella virulence protein that inhibits cellular trafficking. EMBO J 18:3924–33.

    Article  PubMed  CAS  Google Scholar 

  • VanRheenen, S. M., G. Duménil, and R. R. Isberg. 2004. IcmF and DotU are required for optimal effector translocation and trafficking of the Legionella pneumophila vacuole. Infect Immun 72:5972–82.

    Article  PubMed  CAS  Google Scholar 

  • VanRheenen, S. M., Z. Q. Luo, T. O’Connor, and R. R. Isberg. 2006. Members of a Legionella pneumophila family of proteins with ExoU (phospholipase A) active sites are translocated to target cells. Infect Immun 74:3597–606.

    Article  PubMed  CAS  Google Scholar 

  • Vincent, C. D. and J. P. Vogel. 2006. The Legionella pneumophila IcmS-LvgA protein complex is important for Dot/Icm-dependent intracellular growth. Mol Microbiol 61:596–613.

    Article  PubMed  CAS  Google Scholar 

  • Vincent, C. D., Friedman, J. R., Jeong, K. C., Buford, E. C., Miller, J. L., and J. P. Vogel. 2006. Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol [Epub ahead of print].

    Google Scholar 

  • Vogel, J. P., C. Roy, and R. R. Isberg. 1996. Use of salt to isolate Legionella pneumophila mutants unable to replicate in macrophages. Ann NY Acad Sci 797:271–2.

    Article  PubMed  CAS  Google Scholar 

  • Vogel, J. P., H. L. Andrews, S. K. Wong, and R. R. Isberg. 1998. Conjugative transfer by the virulence system of Legionella pneumophila. Science 279:873–6.

    Article  PubMed  CAS  Google Scholar 

  • Watarai, M., H. L. Andrews, and R. R. Isberg. 2001. Formation of a fibrous structure on the surface of Legionella pneumophila associated with exposure of DotH and DotO proteins after intracellular growth. Mol Microbiol 39:313–29.

    Article  PubMed  CAS  Google Scholar 

  • Weber, S. S., C. Ragaz, K. Reus, Y. Nyfeler, and H. Hilbi. 2006. Legionella pneumophila exploits PI(4)P to anchor secreted effector proteins to the replicative vacuole. PLoS Pathog 2:e46.

    Article  PubMed  CAS  Google Scholar 

  • Wiater, L. A., K. Dunn, F. R. Maxfield, and H. A. Shuman. 1998. Early events in phagosome establishment are required for intracellular survival of Legionella pneumophila. Infect Immun 66:4450–60.

    PubMed  CAS  Google Scholar 

  • Zuckman, D. M., J. B. Hung, and C. R. Roy. 1999. Pore-forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growth. Mol Microbiol 32:990–1001.

    Article  PubMed  CAS  Google Scholar 

  • Zusman, T., O. Gal-Mor, and G. Segal. 2002. Characterization of a Legionella pneumophila relA insertion mutant and toles of RelA and RpoS in virulence gene expression. J Bacteriol 184:67–75.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Jason J. LeBlanc
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joseph P. Vogel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer Science+Business Media, LLC

About this chapter

Cite this chapter

LeBlanc, J.J., Vogel, J.P. (2008). The Dot/Icm Type IVB Secretion System. In: Legionella pneumophila. Infectious Diseases And Pathogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-70896-6_3

Download citation

Publish with us

Policies and ethics

Search

Navigation