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Electron Tomography pp 371–399Cite as

Segmentation of Cell Components Using Prior Knowledge

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Abstract

Electron tomography is a method for determining 3D structure by electron microscopy, using multiple tilt views of the specimen (Lucic et al., 2005; McEwen and Marko 2001; McIntosh et al., 2005). Since electron tomography does not employ averaging or require the presence of symmetry, it can be used in biological applications to image single copies of subcellular components in situ. When specimen preparation is optimized by use of rapid freezing, and imaged either directly in the frozen-hydrated state, or after freeze substitution and plastic embedding, electron tomography provides a relatively high-resolution view of biological structure in a native, or near-native, cellular context.

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Authors and Affiliations

  1. Electrical, Computer, and Systems Engineering Department, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA

    Ming Jiang, Qiang Ji & Xun Wang

  2. Resource for Visualization of Biological Complexity, Wadsworth Center, Empire State Plaza, PO Box 509, Albany, NY, 12201-0509, USA

    Bruce F. McEwen

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  1. Ming Jiang
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  2. Qiang Ji
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  4. Bruce F. McEwen
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  1. Wadsworth Center and State University of New York, Albany, NY, 10201, USA

    Joachim Frank

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Jiang, M., Ji, Q., Wang, X., McEwen, B.F. (2007). Segmentation of Cell Components Using Prior Knowledge. In: Frank, J. (eds) Electron Tomography. Springer, New York, NY. https://doi.org/10.1007/978-0-387-69008-7_14

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