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Multi-Photon Molecular Excitation in Laser-Scanning Microscopy

  • Chapter
Handbook Of Biological Confocal Microscopy

Abstract

Multi-photon microscopy (MPM), which is based on molecular excitation by multi-photon absorption (MPA) and is usually combined with laser-scanning microscopy (LSM), has fulfilled its early promise (Denk et al., 1990), as evidenced by continued growth of its application to vital imaging of biological systems (for a recent collection of reprints, see Masters, 2003). Conventional fluorescence microscopy can provide submicron spatial resolution of chemical dynamics within living cells, but is frequently limited in sensitivity and spatial resolution by background due to out-offocus and scattered fluorescence.

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Authors and Affiliations

  1. Max-Planck Institute for Medical Research, Heidelberg, Germany

    Winfried Denk

  2. Molecular Physiology Biophysics, Vanderblt University, Nashville, Tennessee, 37323, USA

    David W. Piston

  3. School of Applied and Engineering Physics, Cornell University, 212 Clark Hall, Ithaca, New York, 14853, USA

    Watt W. Webb

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  1. Winfried Denk
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  2. David W. Piston
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  3. Watt W. Webb
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  1. Department of Zoology, University of Wisconsin, Madison, WI, 53706, USA

    James B. Pawley

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Denk, W., Piston, D.W., Webb, W.W. (2006). Multi-Photon Molecular Excitation in Laser-Scanning Microscopy. In: Pawley, J. (eds) Handbook Of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-45524-2_28

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