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Somatic Cell Nuclear Transfer pp 103–116Cite as

Mitochondrial DNA Inheritance after SCNT

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Part of the Advances in Experimental Medicine and Biology book series (volume 591)

Abstract

Mitochondrial biogenesis and function is under dual genetic control and requires extensive interaction between biparentally inherited nuclear genes and maternally inherited mitochondrial genes. Standard SCNT procedures deprive an oocytes’ mitochondrial DNA (mtDNA) of the corresponding maternal nuclear DNA and require it to interact with an entirely foreign nucleus that is again interacting with foreign somatic mitochondria. As a result, most SCNT embryos, -fetuses, and -offspring carry somatic cell mtDNA in addition to recipient oocyte mtDNA, a condition termed heteroplasmy. It is thus evident that somatic cell mtDNA can escape the selective mechanism that targets and eliminates intraspecific sperm mitochondria in the fertilized oocyte to maintain homoplasmy. However, the factors responsible for the large intra- and interindividual differences in heteroplasmy level remain elusive. Furthermore, heteroplasmy is probably confounded with mtDNA recombination. Considering the essential roles of mitochondria in cellular metabolism, cell signalling, and programmed cell death, future experiments will need to assess the true extent and impact of unorthodox mtDNA transmission on various aspects of SCNT success.

Keywords

  • Nuclear Transfer
  • Somatic Cell Nuclear Transfer
  • Heteroplasmy Level
  • Sperm Mitochondrion
  • Rabbit Oocyte

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Authors and Affiliations

  1. JS Davies Fellow, Department of Animal Science, The University of Adelaide, Roseworthy Campus, Roseworthy, South Australia, 5371, Australia

    Stefan Hiendleder

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  1. Stefan Hiendleder
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Editors and Affiliations

  1. Department of Animal Science, University of Missouri-Columbia, Columbia, Missouri, USA

    Peter Sutovsky Ph.D.

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© 2007 Landes Bioscience and Springer Science+Business Media

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Hiendleder, S. (2007). Mitochondrial DNA Inheritance after SCNT. In: Sutovsky, P. (eds) Somatic Cell Nuclear Transfer. Advances in Experimental Medicine and Biology, vol 591. Springer, New York, NY. https://doi.org/10.1007/978-0-387-37754-4_8

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