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In Situ Reverse Transcription PCR for Detection of mRNA in the CNS

  • Helle Broholm
  • Steen Gammeltoft

Abstract

The development of polymerase chain reaction (PCR) produced a technological breakthrough in nucleic acid detection by increasing molecular sensitivity capabilities. In situ PCR is a marriage of two established technologies: PCR and in situ hybridization based on the amplification within intact cells or tissue sections of specific DNA sequences, or mRNA species, to levels detectable by in situ hybridization and/or immunohistochemistry. Thus, PCR results can be correlated spatially with cell morphology.

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Product Reverse Transcription Polymerase Chain Reaction Acetic Anhydride Polymerase Chain Reaction System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York, Inc. 2002

Authors and Affiliations

  • Helle Broholm
    • 1
  • Steen Gammeltoft
    • 2
  1. 1.Department of NeuropathologyRigshospitalet Copenhagen University HospitalCopenhagen
  2. 2.Department of Clinical BiochemistryGlostrup Hospital, Copenhagen University, GlostrupDenmark, EU

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