In Situ Reverse Transcription PCR for Detection of mRNA in the CNS

  • Helle Broholm
  • Steen Gammeltoft


The development of polymerase chain reaction (PCR) produced a technological breakthrough in nucleic acid detection by increasing molecular sensitivity capabilities. In situ PCR is a marriage of two established technologies: PCR and in situ hybridization based on the amplification within intact cells or tissue sections of specific DNA sequences, or mRNA species, to levels detectable by in situ hybridization and/or immunohistochemistry. Thus, PCR results can be correlated spatially with cell morphology.


Polymerase Chain Reaction Polymerase Chain Reaction Product Reverse Transcription Polymerase Chain Reaction Acetic Anhydride Polymerase Chain Reaction System 
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Copyright information

© Springer-Verlag New York, Inc. 2002

Authors and Affiliations

  • Helle Broholm
    • 1
  • Steen Gammeltoft
    • 2
  1. 1.Department of NeuropathologyRigshospitalet Copenhagen University HospitalCopenhagen
  2. 2.Department of Clinical BiochemistryGlostrup Hospital, Copenhagen University, GlostrupDenmark, EU

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