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Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity

  • Patrizia Aimar
  • Laura Lossi
  • Adalberto Merighi
Chapter

Abstract

We describe a series of techniques based on the use of immunocytochemical labeling methods for the study of the neurochemistry and connectivity of cells within the central nervous system (CNS). According to our current view, neurons communicate to each other mainly at the level of synapses. Although it is now widely accepted that nonsynaptic neuron-to-neuron communication and neuron-to-glia crosstalk also occur, ultrastructural localization of transmitters–modulators at synapses and analysis of neuronal connections still remain major challenges for a correct understanding of the way in which neuronal networks are organized and operate.

Keywords

Immunogold Label Superficial Dorsal Horn Neuronal Connectivity Gold Label Glial Fibrillary Acidic Protein Immunoreactivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York, Inc. 2002

Authors and Affiliations

  • Patrizia Aimar
    • 1
  • Laura Lossi
    • 1
  • Adalberto Merighi
    • 1
    • 2
  1. 1.Department of Veterinary Morphophysiology-Neuroscience Research GroupUniversità degli Studi di TorinoTorinoItaly, EU
  2. 2.Rita Levi Montalcini Center for Brain RepairUniversità degli Studi di TorinoTorinoItaly, EU

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