Analyses of Intracellular Signal Transduction Pathways in CNS Progenitor Cells

  • Elena Cattaneo
  • Luciano Conti


Growth factors such as epidermal growth factor (EGF), fibroblast growth factor (FGF), platelet derived growth factor (PDGF), and the neurotrophins and cytokines, such as interleukines and interferons, have a profound influence on the proliferation, survival, and differentiation of central nervous system (CNS) cells. They exert their roles by binding to their respective membrane-bound receptors and stimulating phosphorylation cascades (4). These receptors have been classified into two major groups: (i) receptors that have an intrinsic tyrosine kinase domain. These are also known as receptor protein tyrosine kinases (RPTK)and are exemplified by the epidermal growth factor receptor (EGFR) and neurotrophin receptors; and (ii) receptors such as those for the interleukins, which lack a kinase domain and use cytoplasmic tyrosine kinases.


Epidermal Growth Factor Receptor Sodium Dodecyl Sulfate Kinase Assay Sodium Orthovanadate Central Nervous System Cell 
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  1. l.
    Bonni, A., Y. Sun, M. Nadal-Vicens, A. Bhatt, D.A. Frank, I. Rozovsky, N. Stahl, G.D. Yancopoulos, and M.E. Greenberg. 1997. Regulation of gliogenesis in the central nervous system by the JAK-STAT signaling pathway. Science 278:477–483.PubMedCrossRefGoogle Scholar
  2. 2.
    Cattaneo, E., L. Magrassi, G. Butti, L. Santi, A. Giavazzi, and S. Pezzotta. 1994. A short term analysis of the behaviour of conditionally immortalized neuronal progenitors and primary neuroepithelial cells implanted into the fetal rat brain. Brain Res. Dev. Brain Res. 83:197–208.PubMedCrossRefGoogle Scholar
  3. 3.
    Cattaneo, E., C. De-Fraja, L. Conti, B. Reinach, L. Bolis, S. Govoni, and E. Liboi. 1996. Activation of the JAK/STAT pathway leads to proliferation of ST14A central nervous system progenitor cells. J. Biol. Chem. 271:23374–23379.PubMedCrossRefGoogle Scholar
  4. 4.
    Cattaneo, E. and P.G. Pelicci. 1998. Emerging roles for SH2/PTB-containing She adaptor proteins in the developing mammalian brain. Trends Neurosci. 21:476–481.PubMedCrossRefGoogle Scholar
  5. 5.
    Cattaneo, E. and L. Conti. 1998. Generation and characterization of embryonic striatal conditionally immortalized ST14A cells. J. Neurosci. Res. 53:223–234.PubMedCrossRefGoogle Scholar
  6. 6.
    Cattaneo, E., L. Conti, and C. De-Fraja. 1999. Signalling through the JAK-STAT pathway in the developing brain. Trends Neurosci. 22:365–369.PubMedCrossRefGoogle Scholar
  7. 7.
    Conti, L., C. De-Fraja, M. Gulisano, E. Migliaccio, S. Govoni, and E. Cattaneo. 1997. Expression and activation of SH2/PTB-containing ShcA adaptor protein reflects the pattern of neurogenesis in the mammalian brain. Proc. Natl. Acad. Sci. USA 94:8185–8190.PubMedCrossRefGoogle Scholar
  8. 8.
    De-Fraja, C., L. Conti, L. Magrassi, S. Govoni, and E. Cattaneo. 1998. Members of the JAK/STAT proteins are expressed and regulated during development in the mammalian forebrain. J. Neurosci. Res. 54:320–330.PubMedCrossRefGoogle Scholar
  9. 9.
    Magrassi, L., M.E. Ehrlich, G. Butti, S. Pezzotta, S. Govoni, and E. Cattaneo. 1998. Basal ganglia precursors found in aggregates following embryonic transplantation adopt a striatal phenotype in heterotopic locations. Development 125:2847–2855.PubMedGoogle Scholar
  10. l0.
    Pawson, T. 1995. Protein modules and signalling networks. Nature 373:573–580.PubMedCrossRefGoogle Scholar
  11. 11.
    Segal, R.A. and M.E. Greenberg. 1996. Intracellular signaling pathways activated by neurotrophic factors. Annu. Rev. Neurosci. 19:463–489.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 2002

Authors and Affiliations

  • Elena Cattaneo
    • 1
  • Luciano Conti
    • 1
  1. 1.Institute of Pharmacological SciencesUniversity of MilanoMilanoItaly, EU

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