GABA is the Main Neurotransmitter Released from Mossy Fiber Terminals in the Developing Rat Hippocampus

  • Victoria F. Safiulina
  • Majid H. Mohajerani
  • Sudhir Sivakumaran
  • Enrico Cherubini
Chapter

Abstract

Early in postnatal development, correlated activity in the hippocampus is characterized by giant depolarizing potentials (GDPs). GDPs are generated by the interplay between glutamate and GABA, which in the immediate postnatal period is depolarizing and excitatory. Here, we review some recent data obtained in our laboratory concerning neuronal signaling at immature MF connections. MF responses were identified on the basis of their strong paired-pulse facilitation, short-term frequency-dependent facilitation and sensitivity to group III mGluR agonist L-AP4. Unlike adulthood, during the first week of postnatal life minimal stimulation of MF evoked responses that were potentiated by flurazepam and abolished by picrotoxin indicating that they were GABAergic. In addition, using a pairing procedure we found that GDPs and associated calcium transients act as coincident detectors for enhancing synaptic efficacy at poorly developed MF-CA3 and MF-interneurons connections. This may be crucial for synaptogenesis and for establishing the adult neuronal circuit.

Keywords

Granule Cell GABAA Receptor Mossy Fiber GABAergic Interneuron Principal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AMPA

α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid

CNS

Central Nervous System

CNQX

6-cyano-7-nitroquinoxaline-2,3-dione

D-APV

D-(-)-2-Amino-5-phosphonopentanoic acid

DCG-IV

(2S,2'R,3'R)-2-(2',3'-Dicarboxycyclopropyl)glycine

DNQX

6,7-Dinitroquinoxaline-2,3-dione

GAT-1

a high-affinity GABA plasma membrane transporter

GABA

γ-Amino-butyric acid

GAD

glutamic acid decarboxylase

GDPs

Giant Depolarizing Potentials

IPSC

inhibitory postsynaptic current

KCC2

neuronal Potassium-Chloride cotransporter

L-AP4

2-amino-4-phosphonobutyric acid

MF

Mossy fibers

mGluR

metabotropic glutamate receptors

NKCC1

Sodium, Potassium Chloride cotransporter

NMDA

N-methyl-D-aspartate

P

postnatal day

VGAT

Vesicular GABA Transporter

Notes

Acknowledgments

The authors are grateful to Drs. A. Kasyanov, G. Fattorini and F. Conti for participating in some experiments. The original research work was supported by grants from Ministero Istruzione, Universita’, Ricerca (MIUR, Italy) and the European Union.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Victoria F. Safiulina
  • Majid H. Mohajerani
  • Sudhir Sivakumaran
  • Enrico Cherubini
    • 1
  1. 1.Neurobiology SectorInternational School for Advanced Studies34014 TriesteItaly

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