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Luminescent Semiconductor Quantum Dots Nanoassemblies for Bioanalysis

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Book cover Reviews in Fluorescence 2004

Part of the book series: Reviews in Fluorescence 2004 ((RFLU,volume 2004))

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Abstract

CdS and CdSe luminescent quantum dots (QDs) have been widely investigated for their luminescence properties 1–20. Luminescent QDs show a number of advantages compared to organic fluorophores commonly used in biological applications. QDs exhibit higher photostability than organic dyes. Their emission band is size dependent due to quantum confinement effects. For example, 3 nm CdSe QDs emit green light while 6 nm CdSe QDs emit red light. It is therefore possible to prepare a series of solutions showing different emission colors by using only one type of semiconductor material. The emission spectra of semiconductor QDs are symmetric and sharp with a full width at half maximum (FWHM) as narrow as 30 nm. On the other hand, emission spectra of organic dyes are asymmetric and broad. This precludes the simultaneous use of several organic dyes to analyze multi-analyte samples due to overlap between their broad emission peaks. Luminescent semiconductor QDs have a wide excitation spectrum which enable the excitation of QDs of different size with a single excitation wavelength. In contrast, multiple excitation wavelengths are needed to simultaneously excite several organic fluorophores. Recognizing their potential bioanalytical researchers have recently applied luminescent QDs as biological labels 21–26, selective ions probes 27 and luminescent gas sensors 28.

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Authors and Affiliations

  1. Department of Chemistry and the Advanced Material Research Institute, University of New Orleans, New Orleans, LA, 70148, USA

    Yongfen Chen & Zeev Rosenzweig

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  1. Yongfen Chen
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  2. Zeev Rosenzweig
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Editors and Affiliations

  1. Institute of Fluorescence, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA

    Chris D. Geddes

  2. Center for Fluorescence Spectroscopy, University of Maryland, Baltimore, Maryland, USA

    Joseph R. Lakowicz

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Chen, Y., Rosenzweig, Z. (2004). Luminescent Semiconductor Quantum Dots Nanoassemblies for Bioanalysis. In: Geddes, C.D., Lakowicz, J.R. (eds) Reviews in Fluorescence 2004. Reviews in Fluorescence 2004, vol 2004. Springer, Boston, MA. https://doi.org/10.1007/978-0-306-48672-2_12

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  • DOI: https://doi.org/10.1007/978-0-306-48672-2_12

  • Publisher Name: Springer, Boston, MA

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