Abstract
We have examined RNA silencing in the blast fungus Magnaporthe grisea using the green fluorescence protein (GFP) gene as a model. The fluorescence in the GFP transformants was efficiently silenced by introducing a plasmid expressing double-stranded GFP RNA. Consequently, GFP mRNA accumulation was drastically reduced in the silenced transformants. In addition, small interfering RNAs of GFP were observed only in the silenced transformants. These results indicate that RNA silencing operates in M. grisea through processes very similar to those reported in other eukaryotes.
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© 2004 Springer Science+Business Media Dordrecht
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Nakayshiki, H., Kadotani, N., Tosa, Y., Mayama, S. (2004). RNA Silencing in the Blast Fungus Magnaporthe Grisea . In: Kawasaki, S. (eds) Rice Blast: Interaction with Rice and Control. Springer, Dordrecht. https://doi.org/10.1007/978-0-306-48582-4_2
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DOI: https://doi.org/10.1007/978-0-306-48582-4_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6268-0
Online ISBN: 978-0-306-48582-4
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