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Fine Genetic Mapping and Physical Delimitation of the Rice Blast Resistance Gene Pi5(t) to a 170-kb DNA Segment of the Rice Genome

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Rice Blast: Interaction with Rice and Control

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Abstract

AFLP-derived markers linked to Pi5(t)-mediated resistance were isolated using bulked segregant analysis of F2 populations generated by crossing three recombinant inbred lines (RILs), RIL125, RIL249, and RIL260 with the susceptible line CO39. The most tightly linked AFLP marker, S04G03, was positioned on chromosome 9 of the fingerprint-based physical map of Nipponbare, a well-characterized rice genotype. Flanking BAC-based Nipponbare markers were generated for saturation mapping using four populations, the three initial RILs and an additional one derived from a cross between M202 and RIL260. A BIBAC (binary BAC) library was constructed from RIL260. Using these resources Pi5(t) was mapped to a 170-kb interval, and a contiguous set of BIBAC clones spanning this region was constructed. It had previously been suggested that Pi3(t) and Pi5(t) might be allelic, due to their identical resistance spectrum and tight linkage. We therefore compared genomic regions for lines containing Pi3(t) using the Pi5(t)-linked markers. DNA gel-blot analyses indicated that the region around Pi3(t) is identical to that of Pi5(t), suggesting that Pi3(t) and Pi5(t) are the same resistance gene.

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Authors and Affiliations

  1. Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University, Yongin, 449-701, Korea

    J.-S. Jeon

  2. DNA LandMarks, Saint-Jean-sur-Richelieu, Quebec, J3B 6Z1, Canada

    D. Chen

  3. National Youngnam Agricultural Experiment Station, Rural Development Administration, Milyang, 627-130, Korea

    G.-H. Yi

  4. Department of Plant Pathology, The Ohio State University, 201 Kottman Hall, 2021 Coffey Road, Columbus, OH, 43210, USA

    G. L. Wang

  5. National Institute of Agrobiological Sciences, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan

    S. Kawasaki

  6. Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA, 95616, USA

    P. C. Ronald

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  1. J.-S. Jeon
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  2. D. Chen
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  3. G.-H. Yi
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  4. G. L. Wang
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  1. National Institute of Agrobiological Sciences, Ibaraki, Japan

    Shinji Kawasaki

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Jeon, JS., Chen, D., Yi, GH., Wang, G.L., Kawasaki, S., Ronald, P.C. (2004). Fine Genetic Mapping and Physical Delimitation of the Rice Blast Resistance Gene Pi5(t) to a 170-kb DNA Segment of the Rice Genome. In: Kawasaki, S. (eds) Rice Blast: Interaction with Rice and Control. Springer, Dordrecht. https://doi.org/10.1007/978-0-306-48582-4_11

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  • DOI: https://doi.org/10.1007/978-0-306-48582-4_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6268-0

  • Online ISBN: 978-0-306-48582-4

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