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Advancing Agrobacterium-Based Crop Transformation and Genome Modification Technology for Agricultural Biotechnology

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Abstract

The last decade has seen significant strides in Agrobacterium-mediated plant transformation technology. This has not only expanded the number of crop species that can be transformed by Agrobacterium, but has also made it possible to routinely transform several recalcitrant crop species including cereals (e.g., maize, sorghum, and wheat). However, the technology is limited by the random nature of DNA insertions, genotype dependency, low frequency of quality events, and variation in gene expression arising from genomic insertion sites. A majority of these deficiencies have now been addressed by improving the frequency of quality events, developing genotype-independent transformation capability in maize, developing an Agrobacterium-based site-specific integration technology for precise gene targeting, and adopting Agrobacterium-delivered CRISPR-Cas genes for gene editing. These improved transformation technologies are discussed in detail in this chapter.

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Notes

Acknowledgements

The work described here was supported by the Applied Science and Technology organization including Vector Construction, Crop Genome Engineering, Controlled Environments, Genomics and Nucleic Acid Analysis at Corteva Agriscience™, Agriculture Division of DowDuPont™. Individual projects were led by Keith Lowe, William Gordon-Kamm, Emily Wu, Ping Che, Jeffery Sander, and Scott Betts.

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Authors and Affiliations

  1. 1.Corteva Agriscience™, Agriculture Division of DowDuPont™JohnstonUSA

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