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Activity-Based Protein Profiling pp 23–48Cite as

Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 420))

Abstract

ABPP methods have been utilized for the last two decades as a means to investigate complex proteomes in all three domains of life. Extensive use in eukaryotes has provided a more fundamental understanding of the biological processes involved in numerous diseases and has driven drug discovery and treatment campaigns. However, the use of ABPP in prokaryotes has been less common, although it has gained more attention over the last decade. The urgent need for understanding bacteriophysiology and bacterial pathogenicity at a foundational level has never been more apparent, as the rise in antibiotic resistance has resulted in the inadequate and ineffective treatment of infections. This is not only a result of resistance to clinically used antibiotics, but also a lack of new drugs and equally as important, new drug targets. ABPP provides a means for which new, clinically relevant drug targets may be identified through gaining insight into biological processes. In this chapter, we place particular focus on the discussion of ABPP strategies that have been applied to study different classes of bacterial enzymes.

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Authors and Affiliations

  1. Department of Chemistry, University of Minnesota-Twin Cities, Minneapolis, MN, 55455, USA

    Shabnam Sharifzadeh & Erin E. Carlson

  2. Department of Medicinal Chemistry, University of Minnesota-Twin Cities, Minneapolis, MN, 55455, USA

    Joshua D. Shirley & Erin E. Carlson

  3. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota-Twin Cities, Minneapolis, MN, 55455, USA

    Erin E. Carlson

Authors
  1. Shabnam Sharifzadeh
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  2. Joshua D. Shirley
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  3. Erin E. Carlson
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Correspondence to Erin E. Carlson .

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Editors and Affiliations

  1. Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA

    Benjamin F. Cravatt

  2. Department of Chemistry, University of Virginia, Charlottesville, VA, USA

    Ku-Lung Hsu

  3. Department of Chemistry, Boston College, Chestnut Hill, MA, USA

    Eranthie Weerapana

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Sharifzadeh, S., Shirley, J.D., Carlson, E.E. (2018). Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles. In: Cravatt, B., Hsu, KL., Weerapana, E. (eds) Activity-Based Protein Profiling. Current Topics in Microbiology and Immunology, vol 420. Springer, Cham. https://doi.org/10.1007/82_2018_135

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