Abstract
Higher plant mitochondria produce nitric oxide (NO) by two separate systems. One is a mitochondrial nitric oxide synthase (NOS), which catalyzes the synthesis of NO and l-citrulline from l-arginine using NAD(P)H. The other one is the respiratory electron transport chain, with the terminal oxidases, CytOx and AOX, which both reduce nitrite to NO. While oxygen is obligatory for the former reaction, the latter activity appears very low in air but high under oxygen deficiency. However, even under anoxia, the rate of nitrite:NO reduction rarely reaches ±1% of respiratory electron transport. For as yet unknown reasons, nitrite:NO reduction appears absent in mitochondria from green leaves. The contribution of NOS and of nitrite reduction to overall NO production, and possible functions of nirite:NO reduction under hypoxia/anoxia are discussed.
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This work was supported by the Deutsche Forschungsgemeinschaft (DFG), SFB 567 and Ka 456-15/1-3.
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Kaiser, W.M., Gupta, K.J., Planchet, E. (2006). Higher Plant Mitochondria as a Source for NO. In: Lamattina, L., Polacco, J.C. (eds) Nitric Oxide in Plant Growth, Development and Stress Physiology. Plant Cell Monographs, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_2006_081
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DOI: https://doi.org/10.1007/7089_2006_081
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