Abstract
Somatic embryogenesis in three important ornamentals is discussed in this chapter. Direct somatic embryo development on the explant tissues (bulb-scale) was noticed in Hippeastrum hybridum and Eucharis grandiflora, both of which are members of Amaryllidaceae. At the time of initiation the embryos were small, water droplet-like, opaque structures, and such development was entirely restricted to the outer rows of scales only. In Crinum asiaticum, callus-mediated (indirect) embryo formation was observed on the flower-bud callus, whereas the bulb-scale callus was largely non-embryogenic. Plant growth regulator, such as 6-benzylaminopurine (BAP), frequently induced somatic embryos in Hippeastrum and Eucharis, and the addition of naphthaleneacetic acid (NAA) further increased the frequency of somatic embryo production. Unlike in many other plant systems, 2,4-D had little or no role in inducing somatic embryogenesis in Hippeastrum and Eucharis. The somatic embryos eventually gave rise to individual plantlets, though occasionally exhibiting dormancy. Histological and scanning electron microscopic observations during the stages of embryo development are presented. The embryo-derived plants had normal chromosome numbers. Besides the importance of true-to-type propagation, somatic embryogenesis offers a system of studying the various facets of non-zygotic embryo development.
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Barbulova A, Iantcheva A, Zhiponova M, Vlahova M, Atanasov A (2002) Establishment of embryogenic potential of economically important Bulgarian alfalfa cultivars (Medicago sativa L.). Biotechnol Biotechnol Equip 16:55–63
Davletova S, Meszaros T, Miskolczi P, Oberschall A, Torok K, Magar Z, Dudits D, Deak M (2001) Auxin and heat shock activation of a novel member of the calmodulin-like domain protein kinase gene family in cultured alfalfa cells. J Exp Bot 52:215–221
Gray DJ, McColley DW, Compton ME (1993) High frequency embryogenesis from quiescent seed cotyledons of Cucumis melo cultivars. J Am Soc Hortic Sci 118:425–432
Iantcheva A, Barbulova A, Vlahova M, Kondorosi E, Elliott M, Atanassov A (1999) Regeneration of diploid annual medics via direct somatic embryogenesis promoted by thidiazuron and benzylaminopurine. Plant Cell Rep 18:904–910
Kysely W, Jacobsen HJ (1990) Somatic embryogenesis from pea embryos and shoot apices. Plant Cell Tissue Organ Cult 20:7–14
Li XQ, Demarly Y (1995) Characterization of factors affecting regeneration frequency of Medicago lupulina L. Euphytica 86:143–148
Maheswaran G, Williams EG (1984) Direct somatic embryoid formation on immature embryos of Trifolium repens, T. pratense and Medicago sativa and rapid clonal propagation of T. repens. Ann Bot 54:201–211
Malik K, Saxena PK (1992) Regeneration in Phaseolus vulgaris L.: High frequency induction of direct shoot formation in intact seedlings by BAP and TDZ. Planta 186:384–388
Meinke DW(1995) Molecular genetics of plant embryogenesis. Annu Rev Plant Physiol Plant Mol Biol 46:369–394
Metheson SL, Nowak J, Maclean N (1990) Selection of regenerative genotypes from highly productive cultivars of alfalfa. Euphytica 45:105–112
Mo LH, Von Arnold S (1991) Origin and development of embryogenic cultures from seedlings of Norway spruce. J Plant Physiol 138:223–230
Mordhorst AP, Toonen MAJ, DeVries SC (1997) Plant embryogenesis. Crit Rev Plant Sci 16:535–576
Mujib A, Bandyopadhyay S, Jana BK, Ghosh PD (1996) Growth regulator involvement and somatic embryogenesis in Crinum asiaticum. Indian J Plant Physiol 1:84–86
Mujib A, Bandyopadhyay S, Jana BK, Ghosh PD (1998) Direct somatic embryogenesis and in vitro plant regeneration in Hippeastrum hybridum. Plant Tissue Cult 8:19–25
Mujib A, Bandyopadhyay S, Ghosh PD (2000) Tissue culture derived plantlet variation in Caladium bicolor L., an important ornamental. Plant Tissue Cult 10:149–155
Murthy B, Murch S, Saxena PK (1995) Thidiazuron-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogea): endogenous growth regulator levels and significance of cotyledons. Physiol Plant 94:268–276
Norgaard JV, Krogstrup P (1991) Cytokinin-induced somatic embryogenesis from immature embryos of Abies nordmanniana Lk. Plant Cell Rep 9:509–513
Pasternak TP, Prinsen E, Ayaydin F, Miskolczi P, Potters G, Asard H, Onckelen HAV, Dudits D, Feher A (2002) The role of auxin, pH and stress in the activation of embryogenic cell division in leaf protoplast derived cells of alfalfa. Plant Physiol 129:1807–1819
Pedroso MC, Pais S (1995) Factors controlling somatic embryogenesis. Plant Cell Tissue Organ Cult 43:147–154
Perry SE, Lehti MD, Fernandez DE (1999) The MADs domain protein AGAMOUS-like 15 accumulates in embryonic tissues with diverse origins. Plant Physiol 120:121–130
Reinert J (1958) Morphogenese und ihre Kontrolle an Gewebekulturen aus Karotten. Naturwissenschaften 45:344–345
Schmidt ED, Guzzo F, Toonen MA, deVries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124:2049–2062
Skene KGM, Barlass M (1983) Studies on the fragmented shoot apex of grapevine. IV. Separation of phenotypes in periclinal chimera in vitro. J Exp Bot 34:1271–1280
Steward FC, Mapes MO, Mears K (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am J Bot 45:705–708
Tabei Y, Kanno T, Nishio T (1991) Regulation of organogenesis and somatic embryogenesis by auxin in melon, Cucumis melo L. Plant Cell Rep 10:225–229
Van Aartrijk J, Vander Linde PCG (1986) In vitro propagation of flower bud crops. In: Zimmerman RH, Griesbach RJ, Hammerschlag FA, Lawson RJ (eds) Tissue culture as a plant production system for horticultural crops. Kluwer, Dordrecht, pp 317–337
Vasil V, Vasil IK (1982) Characterization of an embryogenic cell suspension culture derived from cultured inflorescences of Pennisetum americanum (Pearl millet, Graminae). Am J Bot 699:1441–1449
Wenck AR, Conger BV, Trigiano RN, Sams CE (1988) Inhibition of somatic embryogenesis in orchardgrass by endogenous cytokinins. Plant Physiol 88:990–992
Wernicke W, Milkovits L (1986) Development gradient in wheat leaves. Responses of leaf segments in different genotypes cultured in vitro. J Plant Physiol 115:49–58
Yeung EC (1995) Structural and developmental patterns in somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer, Dordrecht, pp 205–248
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Mujib, A., Banerjee, S., Ghosh, P.D. Origin, Development and Structure of Somatic Embryosin Selected Bulbous Ornamentals: BAP as Inducer. In: Mujib, A., Šamaj, J. (eds) Somatic Embryogenesis. Plant Cell Monographs, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_028
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DOI: https://doi.org/10.1007/7089_028
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