Abstract
Stomatal movement requires large and repetitive changes to cell volume and consequently surface area. These alterations in surface area are accomplished by addition and removal of plasma membrane material. Recent studies of membrane turnover in guard cell protoplasts using electrophysiology and fluorescence imaging techniques implicate that exocytosis and endocytosis are sensitive to changes in membrane tension. This may provide a regulatory mechanism for the adaptation of surface area to osmotically driven changes in cell volume in guard cell protoplasts as well as turgid guard cells. In addition guard cells also exhibit constitutive membrane turnover. Constitutive and tension-driven membrane turnover were found to be associated with addition and removal of K+ channels. This implies that some of the exocytosis and endocytic vesicles carry K+ channels. Together the results demonstrate that exocytosis and endocytosis are essential for stomatal movement and thus gas exchange in plants.
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References
Barth M, Holstein SEH (2004) Identification and functional characterization of Arabidopsis AP180, a binding partner of plant αC-adaptin. J Cell Sci 117:2051–2062
Bick I, Thiel G, Homann U (2001) Cytochalasin D attenuates the desensitisation of pressure-stimulated vesicle fusion in guard cell protoplasts. Eur J Cell Biol 80:521–526
Blatt MR (2000) Cellular signaling and volume control in stomatal movements in plants. Annu Rev Cell Dev Biol 16:221–241
Carroll AD, Moyen C, Van Kesteren P, Tooke F, Battey NH, Brownlee C (1998) Ca2+, annexins, and GTP modulate exocytosis from maize root cap protoplasts. Plant Cell 10:1267–1276
Diekmann W, Hedrich R, Raschke K, Robinson DG (1993) Osmocytosis and vacuolar fragmentation in guard cell protoplasts: their relevance to osmotically-induced volume changes in guard cells. J Exp Bot 44:1569–1577
Franks PJ, Cowan IR, Faquhar GD (1998) A study of stomatal mechanics using the cell pressure probe. Plant Cell Env 21:94–100
Gillis KD (1995) Techniques for membrane capacitance measurements. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum, New York, pp 155–197
Holstein SEH (2002) Clathrin and plant endocytosis. Traffic 3:614–620
Homann U (1998) Fusion and fission of plasma-membrane material accommodates for osmotically induced changes in the surface area of guard-cell protoplasts. Planta 206:329–333
Homann U, Tester M (1998) Patch clamp measurements of capacitance to study exocytosis and endocytosis in plants. Trends Plant Sci 3:110–114
Homann U, Thiel G (1999) Unitary exocytotic and endocytic events in guard-cell protoplasts during osmotic-driven volume changes. FEBS Lett 460:495–499
Homann U, Thiel G (2002) Alteration of K+-channel numbers in the plasma membrane of guard cell protoplasts during surface area changes. Proc Natl Acad Sci USA 99:10215–10220
Hurst AC, Meckel T, Tayefeh S, Thiel G, Homann U (2004) Trafficking of the plant potassium inward rectifier KAT1 in guard cell protoplasts of Vicia faba. Plant J 37:391–397
Kreft M, Zorec R (1997) Cell-attached measurements of attofarad capacitance steps in rat melanotrophs. Pfluegers Arch Eur J Physiol 434:212–214
Kubitscheck U, Homann U, Thiel G (2000) Osmotic evoked shrinking of guard cell protoplasts causes retrieval of plasma membrane into the cytoplasm. Planta 210:423–431
Meckel T, Hurst AC, Thiel G, Homann U (2004) Endocytosis against high turgor: intact guard cells of Vicia faba constitutively endocytose fluorescently labelled plasma membrane and GFP-tagged K+-channel KAT1. Plant J 39:182–193
Meckel T, Hurst AC, Thiel G, Homann U (2005) Guard cells undergo constitutive and pressure driven membrane turnover. Protoplasma (in press)
Morris CE, Homann U (2001) Cell surface area regulation and membrane tension. J Membr Biol 179:79–102
Neher E, Marty M (1982) Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells. Proc Natl Acd Sci USA 21:6712–6716
Raschke K (1979) Movements of stomata. In: Haupt W, Feinleb ME (eds) Encyclopedia of plant physiology, vol 7. Physiology of movements. Springer, Berlin Heidelberg New York, pp 382–441
Shope JC, DeWald DB, Mott KA (2003) Changes in surface area of intact guard cells are correlated with membrane internalization. Plant Physiol 133:1314–21
Thiel G, Rupnik M, Zorec R (1994) Raising the cytosolic Ca2+concentration increases the membrane capacitance of maize coleoptile protoplasts: evidence for Ca2+-stimulated exocytosis. Planta 195:305–308
Thiel G, Sutter J-U, Homann U (2001) Electrophysiological methods: monitoring exo- and endocytosis in real time. In: Hawes C, Satiat-Jeunemaitre B (eds) Plant cell biology. Oxford University Press, Oxford, pp 171–187
Willmer CM, Fricker M (1996) Stomata, 2nd edn. Chapman and Hall, London
Wolfe J, Dowgert MF, Steponkus PL (1986) Mechanical study of the deformation of the plasma membranes of protoplasts during osmotic expansions. J Membr Biol 93:63–74
Zorec R, Tester M (1992) Cytosolic calcium stimulates exocytosis in plant secretory cells. Biophys J 63:864–867
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Homann, U. Endocytosis in Guard Cells. In: Šamaj, J., Baluška, F., Menzel, D. (eds) Plant Endocytosis. Plant Cell Monographs, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7089_016
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DOI: https://doi.org/10.1007/7089_016
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