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Cell Biology and Translational Medicine, Volume 3 pp 53–71Cite as

Challenges in Bio-fabrication of Organoid Cultures

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Part of the book series: Advances in Experimental Medicine and Biology ((CBTMED,volume 1107))

Abstract

Three-dimensional (3D) organoids have shown advantages in cell culture over traditional two-dimensional (2D) culture, and have great potential in various applications of tissue engineering. However, there are limitations in current organoid fabrication technologies, such as uncontrolled size, poor reproductively, and inadequate complexity of organoids. In this chapter, we present the existing techniques and discuss the major challenges for 3D organoid biofabrication. Future perspectives on organoid bioprinting are also discussed, where bioprinting technologies are expected to make a major contribution in organoid fabrication, such as realizing mass production and constructing complex heterotypic tissues, and thus further advance the translational application of organoids in tissue engineering and regenerative medicine as well drug testing and pharmaceutics.

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Abbreviations

2D:

two-dimensional

3D:

three-dimensional

adMSCs:

Adipose-derived mesenchymal stem cells

ASCs:

adipose-derived stem cell

BioLP:

biological laser printing

CXCL:

CXC ligand

CXCR:

CXC receptor

DBB:

droplet-based bioprinting

DPCs:

dental pulp cells

EBB:

extrusion-based bioprinting

ES:

embryonic stem

HA:

hyaluronic acid

HER2:

human epidermal growth receptor

HGF:

hepatocyte growth factor

HIF:

hypoxia-inducible factor

HTC:

hydrogel tissue constructs

HUVECs:

human umbilical vein endothelial cells

LBB:

laser-based bioprinting

MAPK:

mitogen activate protein kinase

MAPLE-DW:

matrix assisted pulsed laser evaporation-direct write

MCS:

multicellular spheroids

MSCs:

mesenchymal stem cells

pHEMA:

poly (2-hydroxethyl methacrylate)

PI3K:

phosphoinositide 3-kinase

PNIPAAm:

poly (N-isopropylacrylamide)

PVA:

polyvinyl alcohol

REF-52:

Rat embryo fibroblasts

RGD:

arginylglycylaspartic acid

SDF:

stromal cell-derived factor

SPIONs:

superparamagnetic iron oxide nanoparticles

TCD:

tissue culture dish

TE:

tissue engineering

TNFα:

tumor necrosis factor

VEGF:

vascular endothelial growth factor

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Acknowledgements

This work has been supported by National Science Foundation Awards # 1624515, National Institutes of Health Grant #R21 CA224422-01A1, an ENGINE grant from Penn State, Diabetes in Action Research and Education Foundation grant # 426, a Wells Fargo grant, the China Scholarship Council 201308360128 and the Oversea Sailing Project from Jiangxi Association for Science and Technology. The authors also acknowledge Indian Council of Medical Research, Government of India, for financial assistance to P.D. The authors are grateful to the support from the Turkish Ministry of National Education for providing graduate scholarship to B.A.

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Authors and Affiliations

  1. Jiangxi Academy of Medical Science, Hospital of Nanchang University, Nanchang, Jiangxi, China

    Weijie Peng

  2. Department of Pharmacology, Nanchang University, Nanchang, Jiangxi, China

    Weijie Peng

  3. Engineering Science and Mechanics Department, Penn State University, University Park, PA, USA

    Weijie Peng, Yang Wu, Bugra Ayan & Ibrahim T. Ozbolat

  4. Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah, West Bengal, India

    Pallab Datta

  5. The Huck Institutes of the Life Sciences, Penn State University, University Park, PA, USA

    Yang Wu, Madhuri Dey, Bugra Ayan & Ibrahim T. Ozbolat

  6. Department of Chemistry, Penn State University, University Park, PA, USA

    Madhuri Dey

  7. Center for Computer-Aided Design, College of Engineering, University of Iowa, Iowa City, IA, USA

    Amer Dababneh

  8. Biomedical Engineering Department, Penn State University, University Park, PA, USA

    Ibrahim T. Ozbolat

  9. Materials Research Institute, Penn State University, University Park, PA, USA

    Ibrahim T. Ozbolat

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  1. Weijie Peng
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  2. Pallab Datta
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  3. Yang Wu
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  5. Bugra Ayan
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  7. Ibrahim T. Ozbolat
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Correspondence to Ibrahim T. Ozbolat .

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  1. Ottawa Hospital Research Institute, Osgoode, ON, Canada

    Kursad Turksen

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Peng, W. et al. (2018). Challenges in Bio-fabrication of Organoid Cultures. In: Turksen, K. (eds) Cell Biology and Translational Medicine, Volume 3. Advances in Experimental Medicine and Biology(), vol 1107. Springer, Cham. https://doi.org/10.1007/5584_2018_216

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